Overview

Easily isolate highly purified and magnetic particle-free human CD4+CD127lowCD25+ regulatory T cells (Tregs) from fresh or previously frozen human peripheral blood mononuclear cells (PBMCs) or leukapheresis samples by immunomagnetic positive selection, with the EasySep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep™ positive selection procedure, desired cells are first labeled with antibody complexes recognizing CD25 and magnetic particles called EasySep™ Releasable RapidSpheres™. Unlike traditional magnetic particles, which stay bound to the target cells, these RapidSpheres™ have a releasable feature. After separation using an EasySep™ magnet, bound magnetic particles are removed from the EasySep™-isolated CD25+ cells using a release agent, and unwanted non-Tregs are targeted for depletion. The final isolated fraction contains highly purified CD4+CD127lowCD25+ cells that express high levels of FOXP3 and are immediately ready for downstream applications. An optional protocol allows for the isolation of CD4+CD25- responder T cells in parallel for use in functional studies. Following cell isolation with this EasySep™ kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins, or other chemically related ligands.

For large-scale isolation of CD4+CD127lowCD25+ Tregs, from leukapheresis samples, see the large-format (1x10¹⁰ cells) kit (Catalog #100-1136).

Magnet Compatibility
- EasySep™ Magnet (Catalog #18000)
- “The Big Easy” EasySep™ Magnet (Catalog #18001)
- EasyEights™ EasySep™ Magnet (Catalog #18103)
- Easy 50 EasySep™ Magnet (Catalog #18002)
- RoboSep™-S (Catalog #21000)
- Easy 250 EasySep™ Magnet (Catalog #100-0821)
 

Subtype
Cell Isolation Kits
 

Cell Type
T Cells, T Cells, CD4+, T Cells, Regulatory
 

Species
Human
 

Sample Source
Leukapheresis, PBMC
 

Selection Method
Positive
 

Application
Cell Isolation
 

Brand
EasySep, RoboSep
 

Area of Interest
Immunology

Product Advantages

- Highly purified human CD4+CD127lowCD25+ Tregs isolated in less than 1 hour

- No-wash removal of EasySep™ Releasable RapidSpheres™

- Optional isolation of CD4+CD25- responder T cells from the same sample

What's Included

- EasySep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit (Catalog #18063)

    - EasySep™ Human CD25 Positive Selection Cocktail, 1 mL

    - EasySep™ Human CD127high Depletion Cocktail, 1 mL

    - EasySep™ Human CD4+ T Cell Enrichment Cocktail, 1 mL

    - EasySep™ Releasable RapidSpheres™, 1 mL

    - EasySep™ Dextran RapidSpheres™, 2 x 1 mL

    - EasySep™ Release Buffer, 2 x 1 mL

- EasySep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit (Catalog #100-1136)

    - EasySep™ Human CD25 Positive Selection Cocktail (1 x 10 mL)

    - EasySep™ Human CD127high Depletion Cocktail (1 x 10 mL)

    - EasySep™ Human CD4+ T Cell Enrichment Cocktail (1 x 10 mL)

    - EasySep™ Releasable RapidSpheres™ 50201 (1 x 10 mL)

    - EasySep™ Dextran RapidSpheres™ 50103 (1 x 5 mL)

    - EasySep™ Release Buffer (Concentrated) (1 x 10 mL)

- RoboSep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit (Catalog #18063RF)

    - EasySep™ Human CD25 Positive Selection Cocktail, 1 mL

    - EasySep™ Human CD127high Depletion Cocktail, 1 mL

    - EasySep™ Human CD4+ T Cell Enrichment Cocktail, 1 mL

    - EasySep™ Releasable RapidSpheres™, 1 mL

    - EasySep™ Dextran RapidSpheres™, 2 x 1 mL

    - EasySep™ Release Buffer, 2 x 1 mL

    - RoboSep™ Buffer (Catalog #20104)

    - RoboSep™ Filter Tips (Catalog #20125) x 2

    - EasySep™ EasyTube™-14 (Catalog #20128)

Catalog #: 18063 (EasySep 1 x 10^9 cells), 18063RF (RoboSep 1 x 10^9 cells), 100-1136 (EasySep 1 x 10^10 cells)

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.