Overview

Efficiently deplete human CD45+ cells from fresh or previously frozen human peripheral blood mononuclear cell (PBMC) samples by immunomagnetic selection, with the EasySep™ Human CD45 Depletion Kit II. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

This straightforward, optimized EasySep™ procedure involves labeling cells with antibody complexes recognizing CD45 and magnetic particles. Labeled cells are separated from untouched cells using an EasySep™ magnet and by simply pouring off the unlabeled cells. The CD45+ cells remain in the tube. Following magnetic cell isolation, desired cells are ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction. The CD45 antigen is expressed on all hematopoietic cells except erythrocytes and platelets.

This product replaces the EasySep™ Human CD45 Depletion Kit (Catalog #18259) for even faster cell isolations.

Magnet Compatibility
- EasySep™ Magnet (Catalog #18000)
- “The Big Easy” EasySep™ Magnet (Catalog #18001)
- RoboSep™-S (Catalog #21000)
 

Subtype
Cell Isolation Kits
 

Cell Type
Cancer Cells and Cell Lines
 

Species
Human
 

Sample Source
PBMC
 

Selection Method
Depletion
 

Application
Cell Isolation
 

Brand
EasySep, RoboSep
 

Area of Interest
Cancer, Immunology, Stem Cell Biology

Product Advantages

- Fast, easy-to-use and column-free

- Up to 4 log depletion of CD45+ cells

- Isolated cells are untouched

What's Included

- EasySep™ Human CD45 Depletion Kit II (Catalog #17898)

    - EasySep™ Human CD45 Depletion Cocktail II, 1 mL

    - EasySep™ Dextran RapidSpheres™, 2 x 1 mL

- RoboSep™ Human CD45 Depletion Kit II (Catalog #17898RF)

    - EasySep™ Human CD45 Depletion Cocktail II, 1 mL

    - EasySep™ Dextran RapidSpheres™, 2 x 1 mL

    - RoboSep™ Buffer (Catalog #20104)

    - RoboSep™ Filter Tips (Catalog #20125)

Catalog #: 17898 (EasySep), 17898RF (RoboSep)

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.