Overview

This kit isolates human CD62L+CD45RO+ and CD62L-CD45RO+ memory CD4+ T cells from fresh peripheral blood mononuclear cells (PBMCs) or washed leukapheresis samples. First, CD62L+ cells are isolated by column-free immunomagnetic positive selection using antibody complexes and EasySep™ Releasable RapidSpheres™. Then, bound magnetic particles are removed from the EasySep™-isolated CD62L+ cells, and unwanted CD45RA+ and non-CD4+ T cells are targeted for depletion using antibody complexes and EasySep™ Dextran RapidSpheres™. The final isolated fraction contains highly purified CD62L+CD45RO+ central memory CD4+ T cells that are immediately ready for downstream applications. Effector memory CD4+ T cells can be isolated from the CD62L- fraction.

Magnet Compatibility
- EasySep™ Magnet (Catalog #18000)
- “The Big Easy” EasySep™ Magnet (Catalog #18001)
- Easy 50 EasySep™ Magnet (Catalog #18002)
- EasyEights™ EasySep™ Magnet (Catalog #18103)
 

Subtype
Cell Isolation Kits
 

Cell Type
T Cells, CD4+
 

Species
Human
 

Sample Source
Leukapheresis, PBMC
 

Selection Method
Positive
 

Application
Cell Isolation
 

Brand
EasySep
 

Area of Interest
Immunology

Product Advantages

- Easy-to-use and column-free

- Up to 96% purity

- No-wash removal of EasySep™ Releasable RapidSpheres™

What's Included

- EasySep™ Human Central and Effector Memory CD4+ T Cell Isolation Kit (Catalog #17865)

    - EasySep™ Human Memory CD4+ T Cell Isolation Cocktail, 0.5 mL

    - EasySep™ Dextran RapidSpheres™, 1 mL

    - EasySep™ Human CD62L Positive Selection Cocktail, 1 mL

    - EasySep Human CD25 Depletion Cocktail, 1 mL

    - EasySep™ Releasable RapidSpheres™, 1 mL

    - EasyeSep™ Release Buffer, 1 mL

Catalog #: 17865

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.