Overview

Isolate highly purified human CD34+ cell from fresh whole umbilical cord blood samples by immunomagnetic positive selection, with the EasySep™ Human Cord Blood CD34 Positive Selection Kit II. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep™ positive selection procedure, hematopoietic progenitor cells are first pre-enriched using the RosetteSep™ Human Cord Blood CD34 Pre-Enrichment Cocktail (15896C) with antibodies recognizing T cell, B cell, myeloid cell, and platelet surface markers. Desired CD34+ cells are then selected using the EasySep™ Human CD34 Positive Selection Cocktail (18096C), which contains an antibody recognizing CD34. The cocktail in this kit also contains an antibody to human Fc receptor to prevent non-specific binding. Labeled cells are separated using an EasySep™ magnet and by simply pouring or pipetting off the unwanted cells. The cells of interest remain in the tube. Following magnetic cell isolation, the desired CD34+ cells are ready for downstream applications.

If isolating CD34+ cells from fresh blood or buffy coat, the Complete Kit for Human Whole Blood CD34+ Cells (Catalog #15086) is recommended.

If isolating CD34+ cells from other samples, including fresh or previously frozen mobilized peripheral blood or bone marrow mononuclear cells, or from previously frozen cord blood mononuclear cells, we recommend using the EasySep™ Human CD34 Positive Selection Kit II (Catalog #17856).

This product replaces the stand-alone EasySep™ Human Cord Blood CD34 Positive Selection Kit (Catalog #18096).

Magnet Compatibility
- EasySep™ Magnet (Catalog #18000)
- “The Big Easy” EasySep™ Magnet (Catalog #18001)
- EasyEights™ EasySep™ Magnet (Catalog #18103)
- RoboSep™-S (Catalog #21000)
 

Subtype
Cell Isolation Kits
 

Cell Type
Hematopoietic Stem and Progenitor Cells
 

Species
Human
 

Sample Source
Cord Blood
 

Selection Method
Positive
 

Application
Cell Isolation
 

Brand
EasySep, RoboSep, RosetteSep
 

Area of Interest
Immunology, Stem Cell Biology

Product Advantages

- Fast and easy-to-use

- Up to 98% purity

- No columns required

- Can be combined with SepMate™ for consistent, high-throughput sample processing

What's Included

- EasySep™ Human Cord Blood CD34 Positive Selection Kit II (Catalog #17896)

    - RosetteSep™ Cord Blood CD34 Pre-Enrichment Cocktail II, 2 x 2.5 mL

    - EasySep™ Human CD34 Positive Selection Cocktail, 2 x 1 mL

    - EasySep™ Dextran RapidSpheres™ 50100, 1 mL

- RoboSep™ Human Cord Blood CD34 Positive Selection Kit II (Catalog #17896RF)

    - RosetteSep™ Cord Blood CD34 Pre-Enrichment Cocktail II, 2 x 2.5 mL

    - EasySep™ Human CD34 Positive Selection Cocktail, 2 x 1 mL

    - EasySep™ Dextran RapidSpheres™ 50100, 1 mL

    - RoboSep™ Buffer (Catalog #20104)

    - RoboSep™ Filter Tips (Catalog #20125) x 2

Catalog #: 17896 (EasySep), 17896RF (RoboSep)

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.