Overview

Isolate highly purified CD34+ cells from fresh whole umbilical cord blood (CB) using a simple, two-step procedure. First, hematopoietic progenitor cells are pre-enriched using the RosetteSep™ Human Progenitor Cell Basic Pre-Enrichment Cocktail (15226C) with antibodies recognizing T cell, B cell and myeloid cell surface markers. CD34+ cells are then selected using the EasySep™ Human CD34 Positive Selection Cocktail (18096C), which contains an antibody recognizing CD34.

This kit does not deplete platelets. If isolating CD34+ cells from whole CB in which platelet contamination is expected the EasySep™ Human Cord Blood CD34 Positive Selection Kit II (Catalog #17896) is recommended.

If isolating CD34+ cells from fresh blood or buffy coat, the Complete Kit for Human Whole Blood CD34+ Cells (Catalog #15086) is recommended.

If isolating CD34+ cells from other samples, including fresh or previously frozen mobilized peripheral blood or bone marrow mononuclear cells, or from previously frozen cord blood mononuclear cells, we recommend using the EasySep™ Human CD34 Positive Selection Kit II (Catalog #17856).

Magnet Compatibility
- EasySep™ Magnet (Catalog #18000)
- “The Big Easy” EasySep™ Magnet (Catalog #18001)
- EasyEights™ EasySep™ Magnet (Catalog #18103)
- RoboSep™ (Catalog #21000)
 

Subtype
Cell Isolation Kits
 

Cell Type
Hematopoietic Stem and Progenitor Cells
 

Species
Human
 

Sample Source
Cord Blood
 

Selection Method
Positive
 

Application
Cell Isolation
 

Brand
EasySep, RoboSep, RosetteSep
 

Area of Interest
Immunology, Stem Cell Biology

Product Advantages

- Fast and easy-to-use

- Up to 96% purity

- No columns required

- Can be combined with SepMate™ for consistent, high-throughput sample processing

What's Included

- EasySep™ Human Cord Blood CD34 Positive Selection Kit III (Catalog #17897)

    - RosetteSep™ Human Progenitor Cell Basic Pre-Enrichment Cocktail, 2 x 2.5 mL

    - EasySep™ Human CD34 Positive Selection Cocktail, 2 x 1 mL

    - EasySep™ Dextran RapidSpheres™ 50100, 1 mL

- RoboSep™ Human Cord Blood CD34 Positive Selection Kit III (Catalog #17897RF)

    - RosetteSep™ Human Progenitor Cell Basic Pre-Enrichment Cocktail, 2 x 2.5 mL

    - EasySep™ Human CD34 Positive Selection Cocktail, 2 x 1 mL

    - EasySep™ Dextran RapidSpheres™ 50100, 1 mL

    - RoboSep™ Buffer (Catalog #20104)

    - RoboSep™ Filter Tips (Catalog #20125)

Catalog #: 17897 (EasySep), 17897RF (RoboSep)

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.