Overview

Efficiently isolate cells expressing epidermal growth factor receptor (EGFR) from fresh or previously frozen human peripheral blood mononuclear cells (PBMCs), leukapheresis products, or cell culture samples in as little as 15 minutes with the EasySep™ Human EGFR Positive Selection Kit. Desired cells are immediately ready for use in downstream applications such as flow cytometry, culture, or DNA/RNA extraction.

Using immunomagnetic EasySep™ technology, EGFR+ cells are labeled with antibodies and magnetic particles and isolated without the use of columns. Labeled cells remain in the tube while unlabeled cells are poured off. Depending on your research needs, either fraction may be used downstream.

This kit targets EGFR, which belongs to the ErbB family of tyrosine kinase receptors (RTK) expressed on the cell surface of circulating tumor cells (CTCs), macrophages, monocytes, plasma cells, and certain T cell subsets, such as effector CD4+ T cells and FoxP3-expressing regulatory CD4+ T cells (Tregs) (Sabbah DA et al. Curr Top Med Chem, 2020 and MacDonald F & Zaiss DMW Front Pharmacol, 2017).

Magnet Compatibility
- EasySep™ Magnet (Catalog #18000)
- “The Big Easy” EasySep™ Magnet (Catalog #18001)
- EasyEights™ EasySep™ Magnet (Catalog #18103)
- EasyEights™ EasySep™ Magnet (Catalog #18103)
- Easy 50 EasySep™ Magnet (Catalog #18002)

Subtype
Cell Isolation Kits
 

Cell Type
Brain Tumor Stem Cells, Cancer Cells and Cell Lines, Macrophages, Monocytes, Mononuclear Cells, T Cells, T Cells, CD4+, Tumor Cells
 

Species
Human
 

Sample Source
Leukapheresis, PBMC
 

Selection Method
Positive

Brand
EasySep
 

Area of Interest
Cancer, Immunology, Cell Therapy Development

Product Advantages

- Fast and easy-to-use

- Up to 98% purity

- No columns required

What's Included

 - EasySep™ Human EGFR Positive Selection Kit ( Catalog #100-1131)

    - EasySep™ Human EGFR Positive Selection Cocktail, 0.5 mL

    - EasySep™ Dextran RapidSpheres™ 50100, 1 x 1 mL

Catalog #: 100-1131

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.