Overview

Efficiently isolate or deplete HLA-DR+ cells from fresh or previously frozen human peripheral blood mononuclear cells (PBMCs) or leukapheresis samples in as little as 25 minutes; and immediately use the desired cells in downstream applications such as flow cytometry, culture, or DNA/RNA extraction.

Use the EasySep™ Human HLA-DR Positive Selection and Depletion Kit to label HLA-DR+ cells with antibodies and magnetic particles. The cells are then subsequently separated using an EasySep™ magnet—without the use of columns. Labeled cells remain in the tube while unlabeled cells are poured off. Depending on your research needs, either fraction may be used downstream.

This kit targets HLA-DR, a human major histocompatibility complex (MHC) class II receptor expressed on the cell surface of antigen-presenting cells (APCs), including dendritic cells, B cells, monocytes, macrophages, and activated T cells (Saraiva et al. Front Immunol, 2018). Research on APCs is relevant to the study of autoimmune diseases, viral infections, and cancer, to mention a few examples.

Magnet Compatibility
- “The Big Easy” EasySep™ Magnet (Catalog #18001)
- EasyEights™ EasySep™ Magnet (Catalog #18103)
- Easy 50 EasySep™ Magnet (Catalog #18002)
- RoboSep™-S (Catalog #21000)
 

Cell Type
B Cells, Dendritic Cells, Macrophages, Monocytes
 

Species
Human
 

Sample Source
Leukapheresis, PBMC
 

Selection Method
Depletion, Positive
 

Application
Cell Isolation
 

Brand
EasySep, RoboSep
 

Area of Interest
Cancer, Immunology, Infectious Diseases, Transplantation Research

Product Advantages

- Fast and easy-to-use
- Up to 98% purity
- No columns required

What's Included

- EasySep™ Human HLA-DR Positive Selection and Depletion Kit (Catalog #100-0980)

    - EasySep™ Human HLA-DR Positive Selection and Depletion Cocktail, 1 x 0.5 mL

    - EasySep™ Dextran RapidSpheres™ 50100, 2 x 1 mL

Catalog #: 100-0980 (EasySep), 100-0982 (RoboSep)

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.