Overview

The EasySep™ Human ILC2 Isolation Kit is designed to isolate Group 2 Innate Lymphoid Cells (ILC2s) from leukapheresis products by immunomagnetic positive selection without the use of a flow sorter.

First, CRTH2+ cells are isolated by column-free immunomagnetic positive selection using antibody complexes and EasySep™ Releasable RapidSpheres™. Then, bound magnetic particles are removed from the EasySep™-isolated CRTH2+ cells, and unwanted non-ILC2s are targeted for depletion using antibody complexes and EasySep™ Dextran RapidSpheres™. The final isolated fraction contains highly purified ILC2s that are immediately ready for downstream applications.

Following cell isolation with this EasySep™ kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins or other chemically related ligands.

Magnet Compatibility
- EasySep™ Magnet (Catalog #18000)
- “The Big Easy” EasySep™ Magnet (Catalog #18001)
 
Subtype
Cell Isolation Kits
 

Cell Type
Innate Lymphoid Cells
 

Species
Human
 

Sample Source
Leukapheresis, PBMC
 

Selection Method
Positive
 

Application
Cell Isolation
 

Brand
EasySep, RoboSep
 

Area of Interest
Immunology

Product Advantages

- Easy-to-use and column-free

- Up to 95% purity

- Facilitates rapid isolation of ILC2s without flow sorting

- No-wash removal of EasySep™ Releasable RapidSpheres™

What's Included

- EasySep™ Human ILC2 Isolation Kit (Catalog #17782)

    - EasySep™ Human CRTH2-PE Positive Selection Cocktail, 0.1 mL

    - EasySep™ Release PE Positive Selection Cocktail, 1 mL

    - EasySep™ Releasable RapidSpheres™ 50201, 1 mL

    - EasySep™ Release Buffer, 1 mL

    - EasySep™ Human ILC2 Isolation Cocktail, 0.5 mL

    - EasySep™ Dextran RapidSpheres™ 50103, 1 mL

    - Anti-Human CD32 Blocker, 1 mL

Catalog #: 17782

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.