Overview

Easily isolate highly purified and magnetic particle-free human memory B cells from fresh or previously frozen human peripheral blood mononuclear cells (PBMCs) or leukapheresis samples, by immunomagnetic positive selection, with the EasySep™ Human Memory B Cell Isolation Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

This EasySep™ positive selection procedure involves labeling CD27+ memory B cells with antibody complexes and EasySep™ Releasable RapidSpheres™. Unlike traditional magnetic particles that stay bound to the target cells, RapidSpheres™ have a releasable feature. Desired cells are first labeled with antibodies and these specialized magnetic particles, before being separated without columns using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Then, bound magnetic particles are removed from the EasySep™-isolated, CD27+ cells using a release agent. Following magnetic cell isolation in as little as 40 minutes with this EasySep™ Release kit, the desired cells are immediately available for downstream applications. Antibody complexes remain bound to the surface of the desired cells and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins or other chemically related ligands.

Magnet Compatibility
- EasySep™ Magnet (Catalog #18000)
- “The Big Easy” EasySep™ Magnet (Catalog #18001)
- Easy 50 EasySep™ Magnet (Catalog #18002)
- EasyEights™ EasySep™ Magnet (Catalog #18103)
 

Subtype
Cell Isolation Kits
 

Cell Type
B Cells
 

Species
Human
 

Sample Source
PBMC
 

Selection Method
Positive
 

Application
Cell Isolation
 

Brand
EasySep
 

Area of Interest
Immunology

Product Advantages

- Highly purified human memory B cells isolated in as little as 33 minutes

- No-wash removal of EasySep™ Releasable RapidSpheres™

- Optional isolation of untouched naïve B cells from the same sample

What's Included

- EasySep™ Human Memory B Cell Isolation Kit (Catalog #17864)

    - EasySep™ Human Memory B Cell Pre-Enrichment Cocktail, 2 x 1 mL

    - EasySep™ Dextran RapidSpheres™, 2 x 1 mL

    - EasySep™ Human CD27 Positive Selection Cocktail, 1 mL

    - EasySep™ Releasable RapidSpheres™, 2 x 1 mL

    - EasySep™ Release Buffer, 2 x 1 mL

Catalog #: 17864

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.