Overview

Easily and efficiently isolate highly purified human (CD14+CD16-) monocytes from fresh or previously frozen human peripheral blood mononuclear cells (PBMCs) or lysed
leukapheresis samples by immunomagnetic negative selection, with the EasySep™ Human Monocyte Enrichment Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep™ negative selection procedure, unwanted cells are labeled with antibody complexes and magnetic particles. The following unwanted cells are targeted for removal: granulocytes, T cells, B cells, dendritic cells, NK cells, and erythroid cells. The magnetically labeled cells are then separated from the untouched desired human monocytes by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube. Following magnetic cell isolation, the desired monocytes are ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction.

For applications in which CD16+ cells are not removed, we recommend the EasySep™ Human Monocyte Enrichment Kit without CD16 Depletion (Catalog #19058).

For even faster cell isolations, we recommend the EasySep™ Human Monocyte Isolation Kit (Catalog #19359), which isolates cells in just 12.5 minutes.

Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• Easy 50 EasySep™ Magnet (Catalog #18002)
• EasyPlate™ EasySep™ Magnet (Catalog 18102)
• RoboSep™-S (Catalog #21000)
 

Subtype
Cell Isolation Kits
 

Cell Type
Monocytes
 

Species
Human
 

Sample Source
PBMC
 

Selection Method
Negative
 

Application
Cell Isolation
 

Brand
EasySep, RoboSep
 

Area of Interest
Immunology

Product Advantages

- Fast, easy-to-use and column-free

- Up to 95% purity

- Untouched, viable cells

What's Included

- EasySep™ Human Monocyte Enrichment Kit (Catalog #19059)

    - EasySep™ Human Monocyte Enrichment Cocktail, 1 mL

    - EasySep™ Magnetic Particles, 1 mL

- RoboSep™ Human Monocyte Enrichment Kit with Filter Tips (Catalog #19059RF)

    - EasySep™ Human Monocyte Enrichment Cocktail, 1 mL

    - EasySep™ Magnetic Particles, 1 mL

    - RoboSep™ Buffer (Catalog #20104)

    - RoboSep™ Filter Tips (Catalog #20125)

Catalog #: 19059 (EasySep), 19059RF (RoboSep)

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.