Overview

Easily and efficiently isolate highly purified human CD14+CD16+ monocytes from fresh or previously frozen human peripheral blood mononuclear cells (PBMCs) or lysed leukapheresis samples by immunomagnetic negative selection, with the EasySep™ Human Monocyte Enrichment Kit without CD16 Depletion. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep™ negative selection procedure, unwanted cells are labeled with antibody complexes and magnetic particles. The following unwanted cells are targeted for removal: granulocytes, T cells, B cells, subsets of dendritic cells, NK cells, and erythroid cells. The cocktail in this kit also contains an antibody to human Fc receptor to prevent non-specific binding. The magnetically labeled cells are then separated from the untouched desired monocytes by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube. Following magnetic cell isolation, the desired monocytes and CD14+CD16+ monocytes are ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction. The CD14+CD16+ subset of monocytes (~10% in blood of healthy individuals) has characteristics of tissue macrophages and expands greatly in acute and chronic inflammatory disease.

For applications in which removal of all CD16+ cells is desired, we recommend the EasySep™ Human Monocyte Enrichment Kit (Catalog #19359), which contains anti-CD16.

For large-scale isolation of CD14+CD16+/- and CD14-CD16+CD56- monocytes from lysed leukapheresis samples, see the large-format (1x10¹⁰ cells) kit (Catalog #100-1525).

Magnet Compatibility
- EasySep™ Magnet (Catalog #18000)
- “The Big Easy” EasySep™ Magnet (Catalog #18001)
- Easy 50 EasySep™ Magnet (Catalog #18002)
- EasyPlate™ EasySep™ Magnet (Catalog 18102)
- RoboSep™-S (Catalog #21000)
- Easy 250 EasySep™ Magnet (Catalog #100-0821)
 

Subtype
Cell Isolation Kits
 

Cell Type
Monocytes
 

Species
Human
 

Sample Source
PBMC
 

Selection Method
Negative
 

Application
Cell Isolation
 

Brand
EasySep, RoboSep
 

Area of Interest
Immunology

Product Advantages

- Fast, easy-to-use, and column-free

- Up to 90% purity

- Untouched, viable cells

What's Included

- EasySep™ Human Monocyte Enrichment Kit without CD16 Depletion (Catalog #19058)

    - EasySep™ Human Monocyte Enrichment Cocktail without CD16 Depletion, 1 mL

    - EasySep™ Magnetic Particles, 1 mL

- EasySep™ Human Monocyte Enrichment Kit without CD16 Depletion (Catalog #100-1525)

    - EasySep™ Human Monocyte Enrichment Cocktail without CD16 Depletion (1 x 10 mL)

    - EasySep™ Magnetic Particles (1 x 10 mL)

- RoboSep™ Human Monocyte Enrichment Kit without CD16 Depletion with Filter Tips (Catalog #19058RF)

    - EasySep™ Human Monocyte Enrichment Cocktail without CD16 Depletion, 1 mL

    - EasySep™ Magnetic Particles, 1 mL

    - RoboSep™ Buffer (Catalog #20104)

    - RoboSep™ Filter Tips (Catalog #20125)

Catalog #: 19058 (EasySep 1 x 10^9 cells), 19058RF (RoboSep 1 x 10^9 cells), 100-1525 (EasySep 1 x 10^10 cells)

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.