Overview

Easily and efficiently isolate highly purified human CD14+CD16- monocytes from fresh or previously frozen human peripheral blood mononuclear cells (PBMCs) or washed leukapheresis samples by immunomagnetic negative selection, with the EasySep™ Human Monocyte Isolation Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep™ negative selection procedure, unwanted cells are labeled with antibody complexes and magnetic particles. The following unwanted cells are targeted for removal: granulocytes, T cells, B cells, dendritic cells, NK cells, and erythroid cells. The magnetically labeled cells are then separated from the untouched desired CD14+CD16- monocytes by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube. Following magnetic cell isolation in as little as 12.5 minutes, the desired monocytes are ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction.

This product replaces EasySep™ Human Monocyte Enrichment Kit (Catalog #19059) for even faster cell isolations with reduced platelet contamination.

For large-scale isolation of CD14+CD16- monocytes from leukapheresis samples, see the large-format (1x10¹⁰ cells) kit (Catalog #100-0697).

Magnet Compatibility
- EasySep™ Magnet (Catalog #18000)
- “The Big Easy” EasySep™ Magnet (Catalog #18001)
- Easy 50 EasySep™ Magnet (Catalog #18002)
- EasyPlate™ EasySep™ Magnet (Catalog #18102)
- EasyEights™ EasySep™ Magnet (Catalog #18103)
- RoboSep™ (Catalog #21000)
- Easy 250 EasySep™ Magnet (Catalog #100-0821)
 

Subtype
Cell Isolation Kits
 

Cell Type
Monocytes
 

Species
Human
 

Sample Source
PBMC
 

Selection Method
Negative
 

Application
Cell Isolation
 

Brand
EasySep, RoboSep
 

Area of Interest
Immunology

Product Advantages

- Fast, easy-to-use and column-free

- Up to 94% purity with high recovery

- Untouched, viable cells

What's Included

- EasySep™ Human Monocyte Isolation Kit (Catalog #19359)

    - EasySep™ Human Monocyte Isolation Cocktail, 1 mL

    - EasySep™ Human Platelet Removal Cocktail, 1 mL

    - EasySep™ D Magnetic Particles for Human Monocytes, 1 mL

- EasySep™ Human Monocyte Isolation Kit (Catalog #100-0697)

    - EasySep™ Human Monocyte Isolation Cocktail, 1 x 10 mL

    - EasySep™ D Magnetic Particles, 1 x 10 mL

- RoboSep™ Human Monocyte Isolation Kit (Catalog #19359RF)

    - EasySep™ Human Monocyte Isolation Cocktail, 1 mL

    - EasySep™ Human Platelet Removal Cocktail, 1 mL

    - EasySep™ D Magnetic Particles for Human Monocytes, 1 mL

    - RoboSep™ Buffer (Catalog #20104)

    - RoboSep™ Filter Tips (Catalog #20125)

Catalog #: 19359 (EasySep 1 x 10^9 cells), 19359RF (RoboSep 1 x 10^9 cells), 100-0697 (EasySep 1 x 10^10 cells)

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.