Overview

Easily and efficiently isolate highly purified human myeloid dendritic cells (mDCs) from fresh human peripheral blood mononuclear cells (PBMCs), buffy coat, or lysed leukapheresis samples by immunomagnetic negative selection, with the EasySep™ Human Myeloid DC Enrichment Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep™ negative selection procedure, unwanted cells are labeled with antibody complexes and magnetic particles. The following unwanted cells are targeted for removal: granulocytes, T cells, B cells, monocytes, non-myeloid dendritic cells, NK cells, erythroid cells, and progenitor cells. The magnetically labeled cells are then separated from the untouched desired mDCs by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube. Following magnetic cell isolation, the desired mDCs are ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction.

Magnet Compatibility
- EasySep™ Magnet (Catalog #18000)
- “The Big Easy” EasySep™ Magnet (Catalog #18001)
- RoboSep™-S (Catalog #21000)
 

Subtype
Cell Isolation Kits
 

Cell Type
Dendritic Cells
 

Species
Human
 

Sample Source
PBMC
 

Selection Method
Negative
 

Application
Cell Isolation
 

Brand
EasySep, RoboSep
 

Area of Interest
Immunology

Product Advantages

- Fast, easy-to-use and column-free

- Up to 90% purity

- Untouched, viable cells

What's Included

- EasySep™ Human Myeloid DC Enrichment Kit (Catalog #19061)

    - EasySep™ Human Myeloid DC Enrichment Cocktail Component A, 2 x 1 mL

    - EasySep™ Human DC Enrichment Cocktail Component B, 2 x 1 mL

    - EasySep™ D Magnetic Particles, 5 x 1 mL

    - Anti-Human CD32 (Fc gamma RII) Blocker, 0.8 mL

- RoboSep™ Human Myeloid DC Enrichment Kit (Catalog #19061RF)

    - EasySep™ Human Myeloid DC Enrichment Cocktail Component A, 2 x 1 mL

    - EasySep™ Human DC Enrichment Cocktail Component B, 2 x 1 mL

    - EasySep™ D Magnetic Particles, 5 x 1 mL

    - Anti-Human CD32 (Fc gamma RII) Blocker, 0.8 mL

    - RoboSep™ Buffer (Catalog #20104) x 2

    - RoboSep™ Filter Tips (Catalog #20125) x 2

Catalog #: 19061 (EasySep), 19061RF (RoboSep)

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.