Overview

Easily and efficiently isolate highly purified human naïve B cells (CD3-CD19+CD27-) from fresh or previously frozen human peripheral blood mononuclear cells (PBMCs) or washed leukapheresis samples by immunomagnetic negative selection, with the EasySep™ Human Naïve B Cell Isolation Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep™ negative selection procedure, unwanted cells are labeled with antibody complexes and magnetic particles. The following unwanted cells are targeted for removal: CD2, CD14, CD16, CD27, CD36, CD43, and glyA. The magnetically labeled cells are then separated from the untouched desired naïve B cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube. Following magnetic cell isolation in as little as 9 minutes, the desired naïve B cells are ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction.

This product can be used in place of the EasySep™ Human Naïve B Cell Enrichment Kit (Catalog #19254) for even faster cell isolations.

Magnet Compatibility
- EasySep™ Magnet (Catalog #18000)
- “The Big Easy” EasySep™ Magnet (Catalog #18001)
- Easy 50 EasySep™ Magnet (Catalog #18002)
- EasyEights™ EasySep™ Magnet (Catalog #18103)
- RoboSep™-S (Catalog #21000)
 

Subtype
Cell Isolation Kits
 

Cell Type
B Cells
 

Species
Human
 

Sample Source
Leukapheresis, PBMC
 

Selection Method
Negative
 

Application
Cell Isolation
 

Brand
EasySep, RoboSep
 

Area of Interest
Immunology

Product Advantages

- Fast, easy-to-use and column-free

- Up to 98% purity with high recovery

- Isolated cells are untouched

What's Included

- EasySep™ Human Naïve B Cell Isolation Kit (Catalog #17254)

    - EasySep™ Human Naïve B Cell Isolation Cocktail, 1 mL

    - EasySep™ Dextran RapidSpheres™, 1 mL

    - EasySep™ Isolation Cocktail Enhancer, 1 mL

- RoboSep™ Human Naïve B Cell Isolation Kit (Catalog #17254RF)

    - EasySep™ Human Naïve B Cell Isolation Cocktail, 1 mL

    - EasySep™ Dextran RapidSpheres™, 1 mL

    - EasySep™ Isolation Cocktail Enhancer, 1 mL

    - RoboSep™ Buffer (Catalog #20104)

    - RoboSep™ Filter Tips (Catalog #20125)

Catalog #: 17254 (EasySep), 17254RF (RoboSep)

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.