Overview

The EasySep™ Human Naïve CD4+ T Cell Isolation Kit is designed to isolate naive CD4+ T cells from fresh or previously frozen peripheral blood mononuclear cells by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes and dextran-coated magnetic particles. CD45RO+ cells are targeted for removal with a biotinylated anti-CD45RO antibody, and a bispecific Tetrameric Antibody Complex that recognizes biotin and dextran. The labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.

For even faster cell isolations, we recommend the new EasySep™ Human Naïve CD4+ T Cell Isolation Kit II (17555) which isolates cells in just 11 minutes.

Magnet Compatibility
- EasySep™ Magnet (Catalog #18000)
- “The Big Easy” EasySep™ Magnet (Catalog #18001)
- Easy 50 EasySep™ Magnet (Catalog #18002)
- EasyEights™ EasySep™ Magnet (Catalog #18103)
- RoboSep™-S (Catalog #21000)
 

Subtype
Cell Isolation Kits
 

Cell Type
T Cells, T Cells, CD4+
 

Species
Human
 

Sample Source
PBMC
 

Selection Method
Negative
 

Application
Cell Isolation
 

Brand
EasySep, RoboSep
 

Area of Interest
Immunology

Product Advantages

- Fast, easy-to-use and column-free

- Up to 96% purity

- Isolated cells are untouched

What's Included

- EasySep™ Human Naïve CD4+ T Cell Isolation Kit (Catalog #19555)

    - EasySep™ Human Naïve CD4+ T Cell Isolation Cocktail, 1 mL

    - EasySep™ Biotinylated Anti-CD45RO Antibody, 1 mL

    - EasySep™ Dextran RapidSpheres™, 1 mL

- RoboSep™ Human Naïve CD4+ T Cell Isolation Kit (Catalog #19555RF)

    - EasySep™ Human Naïve CD4+ T Cell Isolation Cocktail, 1 mL

    - EasySep™ Biotinylated Anti-CD45RO Antibody, 1 mL

    - EasySep™ Dextran RapidSpheres™, 1 mL

RoboSep™ Buffer (Catalog #20104)

RoboSep™ Filter Tips (Catalog #20125)

Catalog #: 19555 (EasySep), 19555RF (RoboSep)

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.