Overview

Easily and efficiently isolate highly purified human naïve CD4+ T cells (CD3+CD4+CD45RA+CD45RO-) from fresh or previously frozen human peripheral blood mononuclear cell (PBMCs) samples by immunomagnetic negative selection, with the EasySep™ Human Naïve CD4+ T Cell Isolation Kit II. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep™ negative selection procedure, unwanted cells are labeled with antibody complexes and magnetic particles. The following unwanted cells are targeted for removal: CD2, CD14, CD16, CD19, CD20, CD25, CD36, CD45RO, CD56, CD61, CD66b, CD123, GlyA, TCRgd, and HLA-DR. The magnetically labeled cells are then separated from the untouched desired naïve CD4+ T cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube. Following magnetic cell isolation in as little as 11 minutes, the desired naïve CD4+ T cells are ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction.

This kit replaces the EasySep™ Human Naïve CD4+ T Cell Enrichment Kit (Catalog #19155) and EasySep™ Human Naïve CD4+ T Cell Isolation Kit (Catalog #19555) for even faster cell isolations.

Magnet Compatibility
- EasySep™ Magnet (Catalog #18000)
- “The Big Easy” EasySep™ Magnet (Catalog #18001)
- Easy 50 EasySep™ Magnet (Catalog #18002)
- EasyEights™ EasySep™ Magnet (Catalog #18103)
- RoboSep™-S (Catalog #21000)
 

Subtype
Cell Isolation Kits
 

Cell Type
T Cells, T Cells, CD4+
 

Species
Human
 

Sample Source
PBMC
 

Selection Method
Negative
 

Application
Cell Isolation
 

Brand
EasySep, RoboSep
 

Area of Interest
Immunology

Product Advantages

- Fast, easy-to-use and column-free

- Up to 98% purity

- Untouched, viable cells

What's Included

- EasySep™ Human Naïve CD4+ T Cell Isolation Kit II (Catalog #17555)

    - EasySep™ Human Naïve CD4+ T Cell Isolation Cocktail II, 1 mL

    - EasySep™ Dextran RapidSpheres™, 1 mL

- RoboSep™ Human Naïve CD4+ T Cell Isolation Kit II (Catalog #17555RF)

    - EasySep™ Human Naïve CD4+ T Cell Isolation Cocktail II, 1 mL

    - EasySep™ Dextran RapidSpheres™, 1 mL

    - RoboSep™ Buffer (Catalog #20104)

    - RoboSep™ Filter Tips (Catalog #20125)

Catalog #: 17555 (EasySep), 17555RF (RoboSep)

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.