Overview

Easily and efficiently isolate highly purified human naïve CD8+ T cells (CD8+CD45RA+CCR7+ and CD45RO-CD57-CD56-) from fresh human peripheral blood mononuclear
cell (PBMCs) samples by immunomagnetic negative selection, with the EasySep™ Human Naïve CD8+ T Cell Isolation Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep™ negative selection procedure, unwanted cells are labeled with antibody complexes and magnetic particles. The following unwanted cells are targeted for removal: CD4, CD14, CD16, CD19, CD20, CD36, CD45RO, CD56, CD57, CD94, CD123, CD244, TCRγ/δ, and glycophorin A. The magnetically labeled cells are then separated from the untouched desired naïve CD8+ T cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube. Following magnetic cell isolation, the desired naïve CD8+ T cells are ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction.

For even faster cell isolations, we recommend the EasySep™ Human Naïve CD8+ T Cell Isolation Kit II (17968) which isolates cells in as little as 11 minutes.

Magnet Compatibility
- EasySep™ Magnet (Catalog #18000)
- “The Big Easy” EasySep™ Magnet (Catalog #18001)
- RoboSep™-S (Catalog #21000)

Subtype
Cell Isolation Kits
 

Cell Type
T Cells, T Cells, CD8+
 

Species
Human
 

Sample Source
PBMC
 

Selection Method
Negative
 

Application
Cell Isolation
 

Brand
EasySep, RoboSep
 

Area of Interest
Immunology

Product Advantages

- Fast, easy-to-use and column-free

- Up to 97% purity

- Untouched, viable cells

What's Included

- EasySep™ Human Naïve CD8+ T Cell Isolation Kit (Catalog #19258)

    - EasySep™ Human Naïve CD8+ T Cell Isolation Cocktail, 1 mL

    - EasySep™ D2 Magnetic Particles, 2 x 1 mL

- RoboSep™ Human Naive CD8+ T Cell Isolation Kit (Catalog #19258RF)

    - EasySep™ Human Naïve CD8+ T Cell Isolation Cocktail, 1 mL

    - EasySep™ D2 Magnetic Particles, 5 x 1 mL

    - RoboSep™ Buffer (Catalog #20104)

    - RoboSep™ Filter Tips (Catalog #20125) x 2

Catalog #: 19258 (EasySep), 19258RF (RoboSep)

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.