Overview

Easily and efficiently isolate highly purified human natural killer (NK) cells from fresh or previously frozen human peripheral blood mononuclear cells (PBMCs) or lysed
leukapheresis samples by immunomagnetic negative selection, with the EasySep™ Human NK Cell Enrichment Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep™ negative selection procedure, unwanted cells are labeled with antibody complexes and magnetic particles. The following unwanted cells are targeted for removal: granulocytes, T cells, B cells, monocytes, dendritic cells, and erythroid cells. The magnetically labeled cells are then separated from the untouched desired human NK cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube. Following magnetic cell isolation, the desired NK cells are ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction.

For even faster cell isolations, we recommend the EasySep™ Human NK Cell Isolation Kit (Catalog #17955) which isolates cells in just 8 minutes.

Magnet Compatibility
- EasySep™ Magnet (Catalog #18000)
- “The Big Easy” EasySep™ Magnet (Catalog #18001)
- Easy 50 EasySep™ Magnet (Catalog #18002)
- EasyPlate™ EasySep™ Magnet (Catalog 18102)
- EasyEights™ EasySep™ Magnet (Catalog #18103)
- RoboSep™-S (Catalog #21000)
 

Subtype
Cell Isolation Kits
 

Cell Type
NK Cells
 

Species
Human
 

Sample Source
PBMC
 

Selection Method
Negative
 

Application
Cell Isolation
 

Brand
EasySep, RoboSep
 

Area of Interest
Immunology

Product Advantages

- Fast, easy-to-use and column-free

- Up to 95% purity

- Untouched, viable cells

What's Included

- EasySep™ Human NK Cell Enrichment Kit (Catalog #19055)

    - EasySep™ Human NK Cell Enrichment Cocktail, 1 mL

    - EasySep™ D Magnetic Particles, 2 x 1 mL

- RoboSep™ Human NK Cell Enrichment Kit with Filter Tips (Catalog #19055RF)

    - EasySep™ Human NK Cell Enrichment Cocktail, 1 mL

    - EasySep™ D Magnetic Particles, 2 x 1 mL

    - RoboSep™ Buffer (Catalog #20104)

    - RoboSep™ Filter Tips (Catalog #20125)

Catalog #: 19055 (EasySep), 19055RF (RoboSep)

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.