Overview

Easily and efficiently isolate highly purified human natural killer (NK) cells from fresh or previously frozen human peripheral blood mononuclear cells (PBMCs) or washed leukapheresis samples by immunomagnetic negative selection, with the EasySep™ Human NK Cell Isolation Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep™ negative selection procedure, unwanted cells are labeled with antibody complexes and magnetic particles. The following unwanted cells are targeted for removal: granulocytes, T cells, B cells, monocytes, dendritic cells, erythroid cells. The magnetically labeled cells are then separated from the untouched desired NK cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube. Following magnetic cell isolation in as little as 8 minutes, the desired NK cells are ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction.

This product replaces EasySep™ Human NK Cell Enrichment Kit (Catalog #19055) for even faster NK cell negative isolation.

For NK cell isolation, including emerging NK cells, we recommend the EasySep™ Human Pan-NK Cell Isolation Kit (Catalog #100-1580), which isolates NK cells containing CD36+ and HLA-DR+ emerging subsets in as little as 8 minutes.

For large-scale isolation of human NK cells from leukapheresis samples, see the large-format (1x10¹⁰ cells) kit (Catalog #100-0960).

Magnet Compatibility
- EasySep™ Magnet (Catalog #18000)
- “The Big Easy” EasySep™ Magnet (Catalog #18001)
- Easy 50 EasySep™ Magnet (Catalog #18002)
- EasyEights™ EasySep™ Magnet (Catalog #18103)
- RoboSep™-S (Catalog #21000)
- Easy 250 EasySep™ Magnet (Catalog #100-0821)
 

Subtype
Cell Isolation Kits
 

Cell Type
NK Cells
 

Species
Human
 

Sample Source
Leukapheresis, PBMC
 

Selection Method
Negative
 

Application
Cell Isolation
 

Brand
EasySep, RoboSep
 

Area of Interest
Chimerism, Immunology

Product Advantages

- Fast, easy-to-use and column-free

- Up to 94% purity

- Isolated cells are untouched

What's Included

- EasySep™ Human NK Cell Isolation Kit (Catalog #17955)

    - EasySep™ Human NK Cell Isolation Cocktail, 1 x 1 mL

    - EasySep™ Dextran RapidSpheres™, 1 x 1 mL

- EasySep™ Human NK Cell Isolation Kit (Catalog #100-0960)

    - EasySep™ Human NK Cell Isolation Cocktail, 1 x 10 mL (Catalog #300-0475)

    - EasySep™ Dextran RapidSpheres™, 1 x 10 mL (Catalog #300-0380)

- RoboSep™ Human NK Cell Isolation Kit (Catalog #17955RF)

    - EasySep™ Human NK Cell Isolation Cocktail, 1 mL

    - EasySep™ Dextran RapidSpheres™, 1 mL

    - RoboSep™ Buffer (Catalog #20104)

    - RoboSep™ Filter Tips (Catalog #20125)

Catalog #: 17955 (EasySep 1 x 10^9 cells), 17955RF (RoboSep 1 x 10^9 cells), 100-0960 (EasySep 1 x 10^10 cells)

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.