Overview

Easily and efficiently isolate highly purified human B cells, including plasma cells, from fresh or previously frozen human peripheral blood mononuclear cells (PBMCs) or lysed
leukapheresis samples by immunomagnetic negative selection, with the EasySep™ Human Pan-B Cell Enrichment Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep™ negative selection procedure, unwanted cells are labeled with antibody complexes and magnetic particles. The following unwanted cells are targeted for removal: CD2, CD3, CD14, CD16, CD36, CD42b, CD56, CD66b, CD123, and glyA. The magnetically labeled cells are then separated from the untouched desired B cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube. Following magnetic cell isolation, the desired B cells are ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction.

For isolation of only CD43-negative B cells from normal samples, we recommend using EasySep™ Human B Cell Enrichment Kit (Catalog #19054).

For enrichment of B cells from peripheral blood or other tissues of patients with B cell leukemia or lymphoma, or with other disease states in which B cells may express CD43, CD36, and/or CD123, we recommend using EasySep™ Human B Cell Enrichment Kit II Without CD43 Depletion (Catalog #17963).

Magnet Compatibility
- EasySep™ Magnet (Catalog #18000)
- “The Big Easy” EasySep™ Magnet (Catalog #18001)
- Easy 50 EasySep™ Magnet (Catalog #18002)
- RoboSep™-S (Catalog #21000)
 

Subtype
Cell Isolation Kits
 

Cell Type
B Cells, Plasma
 

Species
Human
 

Sample Source
Leukapheresis, PBMC
 

Selection Method
Negative
 

Application
Cell Isolation
 

Brand
EasySep, RoboSep
 

Area of Interest
Immunology

Product Advantages

- Fast, easy-to-use and column-free

- Up to 99% purity

- Isolated cells are untouched

What's Included

- EasySep™ Human Pan-B Cell Enrichment Kit (Catalog #19554)

    - EasySep™ Human Pan-B Cell Enrichment Cocktail, 1 mL

    - EasySep™ Magnetic Particles, 2 x 1 mL

- RoboSep™ Human Pan-B Cell Enrichment Kit (Catalog #19554RF)

    - EasySep™ Human Pan-B Cell Enrichment Cocktail, 1 mL

    - EasySep™ Magnetic Particles, 2 x 1 mL

    - RoboSep™ Buffer (Catalog #20104)

    - RoboSep™ Filter Tips (Catalog #20125)

Catalog #: 19554 (EasySep), 19554RF (RoboSep)

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.