Overview

Easily and efficiently isolate highly purified human CD56+ natural killer (NK) cell subsets, including HLA-DR+ and CD36+ emerging populations, from fresh or previously frozen peripheral blood mononuclear cells (PBMCs) or leukapheresis samples. Achieve this isolation through immunomagnetic negative selection with the EasySep™ Human Pan-NK Cell Isolation Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep™ negative selection procedure, unwanted cells are labeled with antibody complexes and magnetic particles. The magnetically labeled cells are then separated from the untouched desired NK cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube. Following magnetic cell isolation in as little as 8 minutes, the desired NK cells are ready for downstream applications, such as flow cytometry, NK cell culture, NK cell expansion, DNA/RNA extraction, and other downstream applications depending on your research needs.

Magnet Compatibility
- EasySep™ Magnet (Catalog #18000)
- “The Big Easy” EasySep™ Magnet (Catalog #18001)
- EasyEights™ EasySep™ Magnet (Catalog #18103)
- Easy 50 EasySep™ Magnet (Catalog #18002)
- RoboSep™-S (Catalog #21000)
 

Cell Type
NK Cells
 

Species
Human
 

Sample Source
Leukapheresis, PBMC
 

Selection Method
Negative
 

Application
Cell Isolation
 

Brand
EasySep
 

Area of Interest
Cancer, Immunology

Product Advantages

- Fast, easy-to-use and column-free
- Up to 96% purity with high recovery
- Isolated cells are untouched

What's Included

- EasySep™ Human Pan-NK Cell Isolation Kit (Catalog #100-1580)
    - EasySep™ Human Pan NK Cell Isolation Cocktail, 1 x 1 mL
    - EasySep™ Dextran RapidSpheres™ 50103, 2 x 1 mL
- RoboSep™ Human Pan-NK Cell Isolation Kit (Catalog #100-1662)
    - EasySep™ Human Pan NK Cell Isolation Cocktail, 1 x 1 mL
    - EasySep™ Dextran RapidSpheres™ 50103, 2 x 1 mL
    - RoboSep™ Filter Tips (Catalog #20125) x 2
    - RoboSep™ Buffer (Catalog #20104)

Catalog #: 100-1580 (EasySep), 100-1662 (RoboSep)

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.