Overview

Easily and efficiently isolate highly purified human plasmacytoid dendritic cells (pDCs) from fresh human peripheral blood mononuclear cells (PBMCs) or leukapheresis samples by immunomagnetic negative selection, with the EasySep™ Human Plasmacytoid DC Isolation Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep™ negative selection procedure, unwanted cells are labeled with antibody complexes and magnetic particles. The magnetically labeled cells are then separated from the untouched desired pDCs by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube. Following magnetic cell isolation, the desired pDCs are ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction.

This product replaces the EasySep™ Human Plasmacytoid DC Enrichment Kit (Catalog #19062) for even faster cell isolations and is compatible with a greater number of EasySep™ magnets.

Magnet Compatibility
- EasySep™ Magnet (Catalog #18000)
- “The Big Easy” EasySep™ Magnet (Catalog #18001)
- EasyEights™ EasySep™ Magnet (Catalog #18103)
- Easy 50 EasySep™ Magnet (Catalog #18002)
- RoboSep™-S (Catalog #21000)
 

Subtype
Cell Isolation Kits
 

Cell Type
Dendritic Cells
 

Species
Human
 

Sample Source
Leukapheresis, PBMC
 

Selection Method
Negative
 

Application
Cell Isolation
 

Brand
EasySep, RoboSep
 

Area of Interest
Immunology, Infectious Diseases

Product Advantages

- Fast, easy-to-use, and column-free

- Up to 97% purity

- Untouched, viable cells

What's Included

- EasySep™ Human Plasmacytoid DC Isolation Kit (Catalog #17977)

    - EasySep™ Human Plasmacytoid DC Isolation Cocktail Component A, 2 x 1 mL

    - EasySep™ Human Plasmacytoid DC Isolation Cocktail Component B, 2 x 1 mL

    - EasySep™ Dextran RapidSpheres™ 50103, 4 x 1 mL

    - Anti-Human CD32 (Fc gamma RII) Blocker, 2 x 0.8 mL

Catalog #: 17977 (EasySep), 17977RF (RoboSep)

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.