Overview

Easily and efficiently isolate highly purified human hematopoietic progenitor cells from fresh or previously frozen human cord blood or other cell preparations that contain large numbers of platelets by immunomagnetic negative selection, with the EasySep™ Human Progenitor Cell Enrichment Kit with Platelet Depletion. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep™ negative selection procedure, unwanted cells are labeled with antibody complexes and magnetic particles. The following unwanted cells are targeted for removal: CD2, CD3, CD11b, CD11c, CD14, CD16, CD19, CD24, CD56, CD61, CD66b, and glyA. The magnetically labeled cells are then separated from the untouched desired hematopoietic progenitor cells by using an EasySep™ magnet and simply pouring the desired cells into a new tube. Following magnetic cell isolation, the desired hematopoietic progenitor cells are ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction.

Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• RoboSep™-S (Catalog #21000)
 

Subtype
Cell Isolation Kits
 

Cell Type
Hematopoietic Stem and Progenitor Cells
 

Species
Human
 

Sample Source
Cord Blood, PBMC
 

Selection Method
Negative
 

Application
Cell Isolation
 

Brand
EasySep, RoboSep
 

Area of Interest
Immunology, Stem Cell Biology

Product Advantages

- Fast, easy-to-use and column-free

- Up to 75% purity

- Isolated cells are untouched

What's Included

- EasySep™ Human Progenitor Cell Enrichment Kit with Platelet Depletion (Catalog #19356)

    - EasySep™ Human Progenitor Cell Enrichment Cocktail with Platelet Depletion, 1 mL

    - EasySep™ Magnetic Particles, 2 x 1 mL

- RoboSep™ Human Progenitor Enrichment with Platelet Depletion (Catalog #19356RF)

    - EasySep™ Human Progenitor Cell Enrichment Cocktail with Platelet Depletion, 1 mL

    - EasySep™ Magnetic Particles, 2 x 1 mL

    - RoboSep™ Buffer (Catalog #20104)

    - RoboSep™ Filter Tips (Catalog #20125)

Catalog #: 19356 (EasySep), 19356RF (RoboSep)

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.