Overview

Easily isolate highly purified and magnetic particle-free human Th17 (CD4+CXCR3-CCR6+) cells from fresh human peripheral blood mononuclear cells (PBMCs) or leukapheresis samples by immunomagnetic positive selection, with the EasySep™ Human Th17 Enrichment Kit II. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep™ positive selection procedure, desired cells are first labeled with antibody complexes recognizing CCR6 and magnetic particles called EasySep™ Releasable RapidSpheres™. Unlike traditional magnetic particles, which stay bound to the target cells, these RapidSpheres™ have a releasable feature. After separation using an EasySep™ magnet, bound magnetic particles are removed from the EasySep™-isolated CCR6+ cells using a release agent, and unwanted non-CD4+ T cells, CD45RA+ cells, and CXCR3+ cells are targeted for depletion. The final isolated fraction contains highly purified IL-17-producing Th17 cells, which are immediately ready for downstream applications.

This product can be used in place of the EasySep™ Human Th17 Cell Enrichment Kit (Catalog #18162) for even faster cell isolations.

Subtype
Cell Isolation Kits
 

Cell Type
T Cells, CD4+
 

Species
Human
 

Sample Source
Leukapheresis, PBMC
 

Selection Method
Positive
 

Application
Cell Isolation
 

Brand
EasySep
 

Area of Interest
Immunology

Product Advantages

- Fast and easy-to-use

- Up to 98% purity

- No columns required

What's Included

- EasySep™ Human Th17 Enrichment Kit II (Catalog #17862)

    - EasySep™ Human CCR6 Positive Selection Cocktail II, 0.5 mL

    - EasySep™ Releasable RapidSpheres™, 4 x 1 mL

    - EasySep™ Human CD4+CXCR3- T Cell Pre-Enrichment Cocktail, 1 mL

    - EasySep™ Dextran RapidSpheres™, 1 mL

    - EasySep™ Release Buffer, 2 x 1 mL

Catalog #: 17862

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.