Overview

Isolate highly purified human CD138+ (syndecan-1) cells from fresh bone marrow or whole blood samples by immunomagnetic positive selection, with the EasySep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Kit II. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep™ positive selection procedure, desired cells are labeled with antibody complexes recognizing CD138+ (syndecan-1) and dextran-coated magnetic particles. The cocktail in this kit also contains an antibody to human Fc receptor to prevent non-specific binding. Labeled cells are separated using an EasySep™ magnet and by simply pouring or pipetting off the unwanted cells. The cells of interest remain in the tube. Following magnetic cell isolation, the desired CD138+ cells are ready for downstream applications such as fluorescence in situ hybridization (FISH), flow cytometry, culture, or DNA/RNA extraction. The CD138 antigen is expressed on normal and malignant plasma cells (but not mature B cells).

This product replaces the EasySep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Kit (Catalog #18387) for even faster cell isolations.

Take our free On-Demand CD138+ Cell Isolation Course to learn how to process bone marrow samples and isolate CD138+ plasma cells to increase sensitivity in FISH testing for multiple myeloma. You can also browse our Frequently Asked Questions (FAQs) about isolating CD138+ plasma cells to improve FISH sensitivity.

Magnet Compatibility
- “The Big Easy” EasySep™ Magnet (Catalog #18001)
- EasyEights™ EasySep™ Magnet (Catalog #18103)
- RoboSep™-S (Catalog #21000)
 

Subtype
Cell Isolation Kits
 

Cell Type
Myeloma, Plasma
 

Species
Human
 

Sample Source
Bone Marrow, Whole Blood
 

Selection Method
Positive
 

Application
ELISA
 

Brand
EasySep, RoboSep
 

Area of Interest
Immunology

Product Advantages

- Fast and easy-to-use

- No columns required

What's Included

- EasySep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Kit II (Catalog #17887)

    - EasySep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Cocktail, 3 x 1 mL

    - EasySep™ Dextran RapidSpheres™ 50100, 3 x 1 mL

    - EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)

- RoboSep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Kit II (Catalog #17887RF)

    - EasySep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Cocktail, 3 x 1 mL

    - EasySep™ Dextran RapidSpheres™ 50100, 3 x 1 mL

    - EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)

    - RoboSep™ Buffer (Catalog #20104) x 2

    - RoboSep™ Filter Tips (Catalog #20125) x 2

Catalog #: 17887 (EasySep), 17887RF (RoboSep)

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.