Overview

Isolate highly purified mouse CD138+ cells from mouse splenocytes, lymph nodes, or bone marrow samples by immunomagnetic positive selection, with the EasySep™ Mouse CD138 Positive Selection Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep™ positive selection procedure, desired cells are labeled with antibody complexes recognizing CD138 and magnetic particles. Labeled cells are separated using an EasySep™ magnet and by simply pouring or pipetting off the unwanted cells. The cells of interest remain in the tube. Following magnetic cell isolation, the desired CD138+ cells are ready for downstream applications such as flow cytometry, cell culture, and hybridoma generation.

Magnet Compatibility
- EasySep™ Magnet (Catalog #18000)
- “The Big Easy” EasySep™ Magnet (Catalog #18001)
- EasyEights™ EasySep™ Magnet (Catalog #18103)
- RoboSep™-S (Catalog #21000)
 

Subtype
Cell Isolation Kits
 

Cell Type
B Cells, Plasma
 

Species
Mouse
 

Sample Source
Bone Marrow, Lymph Node, Other, Spleen
 

Selection Method
Positive
 

Application
Cell Isolation
 

Brand
EasySep, RoboSep
 

Area of Interest
Immunology

Product Advantages

- Fast, easy-to-use and column-free

- Up to 97% purity with immunized mice

- Useful for the enrichment of plasma cells

- Compatible with hybridoma generation protocols, including electrofusion

What's Included

- EasySep™ Mouse CD138 Positive Selection Kit (Catalog #18957)

    - EasySep™ Mouse CD138 Positive Selection Cocktail, 1 mL

    - EasySep™ Dextran RapidSpheres™ 50100, 1 mL

    - EasySep™ Mouse FcR Blocker, 2 x 0.5 mL

- RoboSep™ Mouse CD138 Positive Selection Kit (Catalog #18957RF)

    - EasySep™ Mouse CD138 Positive Selection Cocktail, 1 mL

    - EasySep™ Dextran RapidSpheres™ 50100, 1 mL

    - EasySep™ Mouse FcR Blocker, 2 x 0.5 mL

    - RoboSep™ Buffer (Catalog #20104)

    - RoboSep™ Filter Tips (Catalog #20125)

    - EasySep™ EasyTube™-14 (Catalog #20128) x 2

Catalog #: 18957 (EasySep), 18957RF (RoboSep)

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.