Overview

Easily isolate highly purified mouse CD19+ cells from single-cell suspensions of mouse splenocytes or other tissue samples using immunomagnetic positive selection with the EasySep™ Mouse CD19 Positive Selection Kit II. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep™ positive selection procedure, desired cells are labeled with antibody complexes recognizing CD19 and magnetic particles. Labeled cells are separated using an EasySep™ magnet and by simply pouring or pipetting off the unwanted cells. The cells of interest remain in the tube. Following magnetic cell isolation, the desired CD19 cells are ready for downstream applications such as flow cytometry, culture, and cell-based experiments.

Magnet Compatibility
- EasySep™ Magnet (Catalog #18000)
- “The Big Easy” EasySep™ Magnet (Catalog #18001)
- EasyEights™ EasySep™ Magnet (Catalog #18103)
- RoboSep™-S (Catalog #21000)
 

Subtype
Cell Isolation Kits
 

Cell Type
B Cells
 

Species
Mouse
 

Sample Source
Other, Spleen
 

Selection Method
Positive
 

Application
Cell Isolation
 

Brand
EasySep, RoboSep
 

Area of Interest
Immunology

Product Advantages

- Fast and easy-to-use

- Up to 99% purity

- No columns required

- Isolated cells are not fluorochrome-labeled

What's Included

- EasySep™ Mouse CD19 Positive Selection Kit II (Catalog #18954)

    - EasySep™ Mouse CD19 Positive Selection II Component A, 0.5 mL

    - EasySep™ Mouse CD19 Positive Selection II Component B, 0.5 mL

    - EasySep™ Dextran RapidSpheres™ 50100, 2 x 1 mL

    - RoboSep™ Empty Vial

- RoboSep™ Mouse CD19 Positive Selection Kit II (Catalog #18954RF)

    - EasySep™ Mouse CD19 Positive Selection II Component A, 0.5 mL

    - EasySep™ Mouse CD19 Positive Selection II Component B, 0.5 mL

    - EasySep™ Dextran RapidSpheres™ 50100, 2 x 1 mL

    - RoboSep™ Empty Vial

    - RoboSep™ Buffer (Catalog #20104)

    - RoboSep™ Filter Tips (Catalog #20125) x 2

Catalog #: 18954 (EasySep), 18954RF (RoboSep)

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.