Overview

Isolate highly purified mouse CD4+ cells from single-cell suspension of mouse splenocytes or other tissue samples with ease, using immunomagnetic positive selection and the EasySep™ Mouse CD4 Positive Selection Kit II. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep™ positive selection procedure, desired cells are labeled with antibody complexes recognizing CD4 and magnetic particles. Labeled cells are separated using an EasySep™ magnet and by simply pouring or pipetting off the unwanted cells. The cells of interest remain in the tube. Following magnetic cell isolation, the desired CD4+ cells are ready for downstream applications such as flow cytometry, culture, and cell-based experiments.

Magnet Compatibility
- EasySep™ Magnet (Catalog #18000)
- “The Big Easy” EasySep™ Magnet (Catalog #18001)
- EasyEights™ EasySep™ Magnet (Catalog #18103)
- RoboSep™-S (Catalog #21000)
 

Subtype
Cell Isolation Kits
 

Cell Type
T Cells, T Cells, CD4+
 

Species
Mouse
 

Sample Source
Other, Spleen
 

Selection Method
Positive
 

Application
Cell Isolation
 

Brand
EasySep, RoboSep
 

Area of Interest
Immunology

Product Advantages

- Fast, easy-to-use, and column-free

- Up to 98% purity

- Isolated cells are not fluorochrome-labeled

What's Included

- EasySep™ Mouse CD4 Positive Selection Kit II (Catalog #18952)

    - EasySep™ Mouse CD4 Positive Selection II Component A, 0.5 mL

    - EasySep™ Mouse CD4 Positive Selection II Component B, 0.5 mL

    - Mouse FcR PolyBlock (Catalog #300-0902), 1.2 mL

    - EasySep™ Dextran RapidSpheres™ 50100, 1 mL

- RoboSep™ Mouse CD4 Positive Selection Kit II (Catalog #18952RF)

    - EasySep™ Mouse CD4 Positive Selection II Component A, 0.5 mL

    - EasySep™ Mouse CD4 Positive Selection II Component B, 0.5 mL

    - Mouse FcR PolyBlock (Catalog #300-0902), 1.2 mL

    - EasySep™ Dextran RapidSpheres™ 50100, 1 mL

    - RoboSep™ Empty Vial

    - RoboSep™ Buffer (Catalog #20104)

    - RoboSep™ Filter Tips (Catalog #20125) x 2

Catalog #: 18952 (EasySep), 18952RF (RoboSep)

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.