Overview

Isolate highly purified naïve CD4+ T cells from single-cell suspensions of splenocytes or other tissues by using a simple, two-step method. First, naive CD4+ T cells are pre-enriched by negative selection using the EasySep™ Mouse Naïve CD4+ T Cell Pre-Enrichment Cocktail containing biotinylated antibodies directed against non-naïve CD4+ T cells (CD8, CD11b, CD11c, CD19, CD24, CD25, CD45R, CD49b, TCRγ/δ, TER119). Then CD4+CD62L+ cells, pre-labeled with CD62L PE, are selected using the EasySep™ PE Selection Cocktail. The desired cells are labeled with antibodies and magnetic particles. The cells are separated without columns using an EasySep™ magnet.

For the isolation of mouse naïve CD4+ T cells in as little as 15 minutes by negative selection, we recommend using the EasySep™ Mouse Naïve CD4+ T Cell Isolation Kit (19765).

Magnet Compatibility
- EasySep™ Magnet (Catalog #18000)
- “The Big Easy” EasySep™ Magnet (Catalog #18001)
- RoboSep™-S (Catalog #21000)
 

Subtype
Cell Isolation Kits
 

Cell Type
T Cells, T Cells, CD4+
 

Species
Mouse
 

Sample Source
Other, Spleen
 

Selection Method
Positive
 

Application
Cell Isolation
 

Brand
EasySep, RoboSep
 

Area of Interest
Immunology

Product Advantages

- Fast and easy-to-use

- Up to 97% purity

- No columns required

What's Included

- EasySep™ Mouse CD4+CD62L+ T Cell Isolation Kit (Catalog #18765)

    - EasySep™ Mouse Naïve CD4+ T Cell Pre-Enrichment Cocktail, 0.5 mL

    - EasySep™ Streptavidin RapidSpheres™ 50001, 1 mL

    - EasySep™ PE Positive Selection Cocktail, 1 mL

    - EasySep™ Dextran RapidSpheres™ 50100, 1 mL

    - EasySep™ Mouse FcR Blocker (Catalog #18720), 0.1 mL

- RoboSep™ Mouse CD4+CD62L+ T Cell Isolation Kit (Catalog #18765RF)

    - EasySep™ Mouse Naïve CD4+ T Cell Pre-Enrichment Cocktail, 0.5 mL

    - EasySep™ Streptavidin RapidSpheres™ 50001, 1 mL

    - EasySep™ PE Positive Selection Cocktail, 1 mL

    - EasySep™ Dextran RapidSpheres™ 50100, 1 mL

    - EasySep™ Mouse FcR Blocker (Catalog #18720), 0.1 mL

    - RoboSep™ Buffer (Catalog #20104)

    - RoboSep™ Filter Tips (Catalog #20125) x 2

Catalog #: 18765 (EasySep), 18765RF (RoboSep)

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.