Overview

The EasySep™ Mouse Memory CD4+ T Cell Isolation Kit is designed to isolate pan-memory CD4+ T cells from single-cell suspensions of splenocytes or other tissues by negative selection. Unwanted cells are targeted for removal with biotinylated antibodies directed against non-memory CD4+ T cells (CD8, CD11b, CD11c, CD19, CD24, CD45R, CD45RB, CD49b, CD105 and TCRγ/δ) and streptavidin-coated magnetic particles (RapidSpheres™ ). Labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.

Magnet Compatibility
- EasySep™ Magnet (Catalog #18000)
- “The Big Easy” EasySep™ Magnet (Catalog #18001)
- EasyEights™ EasySep™ Magnet (Catalog #18103)
- RoboSep™-S (Catalog #21000)
 

Subtype
Cell Isolation Kits
 

Cell Type
T Cells, T Cells, CD4+
 

Species
Mouse
 

Sample Source
Other, Spleen
 

Selection Method
Negative
 

Application
Cell Isolation
 

Brand
EasySep, RoboSep
 

Area of Interest
Immunology

Product Advantages

- Fast, easy-to-use and column-free

- Up to 96% purity

- Untouched, viable cells

What's Included

- EasySep™ Mouse Memory CD4+ T Cell Isolation Kit (Catalog #19767)

    - EasySep™ Mouse Memory CD4+ T Cell Isolation Cocktail, 0.5 mL

    - EasySep™ Streptavidin RapidSpheres™ 50001, 2 x 1 mL

    - EasySep™ Mouse FcR Blocker (Catalog #18730), 0.2 mL

- RoboSep™ Mouse Memory CD4+ T Cell Isolation Kit (Catalog #19767RF)

    - EasySep™ Mouse Memory CD4+ T Cell Isolation Cocktail, 0.5 mL

    - EasySep™ Streptavidin RapidSpheres™ 50001, 2 x 1 mL

    - EasySep™ Mouse FcR Blocker (Catalog #18730), 0.2 mL

    - RoboSep™ Buffer (Catalog #20104)

    - RoboSep™ Filter Tips (Catalog #20125)

Catalog #: 19767 (EasySep), 19767RF (RoboSep)

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.