Overview

Easily and efficiently isolate highly purified mouse monocytes from mouse bone marrow, splenocytes, whole blood, or other single-cell suspension samples by immunomagnetic negative selection, with the EasySep™ Mouse Monocyte Isolation Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep™ negative selection procedure, unwanted cells are labeled with antibody complexes and magnetic particles. The following unwanted cells are targeted for removal: granuloctyes, T cells, B cells, NK cells, hematopoietic progenitor cells, and erythroid cells. The magnetically labeled cells are then separated from the untouched desired monocytes by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube. Following magnetic cell isolation in as little as 15 minutes, the desired monocytes are ready for downstream applications such as flow cytometry, culture, or cell-based assays.

Magnet Compatibility
- EasySep™ Magnet (Catalog #18000)
- “The Big Easy” EasySep™ Magnet (Catalog #18001)
- EasyEights™ EasySep™ Magnet (Catalog #18103)
- EasyPlate™ EasySep™ Magnet (Catalog #18102)
- RoboSep™-S (Catalog #21000)
 

Subtype
Cell Isolation Kits
 

Cell Type
Monocytes
 

Species
Mouse
 

Sample Source
Bone Marrow, Spleen, Whole Blood
 

Selection Method
Negative
 

Application
Cell Isolation

Brand
EasySep, RoboSep
 

Area of Interest
Immunology

Product Advantages

- Fast and easy-to-use

- Up to 95% purity

- No columns required

- Untouched, viable cells

What's Included

- EasySep™ Mouse Monocyte Isolation Kit (Catalog #19861)

    - EasySep™ Mouse Monocyte Isolation Cocktail Component A, 0.5 mL

    - EasySep™ Mouse Monocyte Isolation Cocktail Component B, 0.5 mL

    - EasySep™ Dextran RapidSpheres™ 50103, 1 mL

    - EasySep™ Mouse FcR Blocker (Catalog #18731), 0.5 mL

- RoboSep™ Mouse Monocyte Isolation Kit (Catalog #19861RF)

    - EasySep™ Mouse Monocyte Isolation Cocktail Component A, 0.5 mL

    - EasySep™ Mouse Monocyte Isolation Cocktail Component B, 0.5 mL

    - EasySep™ Dextran RapidSpheres™ 50103, 1 mL

    - EasySep™ Mouse FcR Blocker (Catalog #18731), 0.5 mL

    - RoboSep™ Empty Vial

    - RoboSep™ Buffer (Catalog #20104)

    - RoboSep™ Filter Tips (Catalog #20125)

Catalog #: 19861 (EasySep), 19861RF (RoboSep)

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.