Overview

Easily and efficiently isolate highly purified mouse naïve CD8+ T cells (CD3+CD8+CD44-CD62L+) from single-cell suspension of mouse splenocytes or lymph node samples by immunomagnetic negative selection, with the EasySep™ Mouse Naïve CD8+ T Cell Isolation Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep™ negative selection procedure, unwanted cells are labeled with antibody complexes and magnetic particles. The following unwanted cells are targeted for removal: CD4, CD11b, CD11c, CD19, CD44, CD45R/B220, CD49b, TCRγ/δ, and TER119. The magnetically labeled cells are then separated from the untouched desired naïve CD8+ T cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube. Following magnetic cell isolation in as little as 17.5 minutes, the desired naïve CD8+ T cells are ready for downstream applications such as flow cytometry, culture, and cell-based experiments.

Magnet Compatibility
- EasySep™ Magnet (Catalog #18000)
- “The Big Easy” EasySep™ Magnet (Catalog #18001)
- EasyPlate™ EasySep™ Magnet (Catalog 18102)
- RoboSep™-S (Catalog #21000)
 

Subtype
Cell Isolation Kits
 

Cell Type
T Cells, T Cells, CD8+
 

Species
Mouse
 

Sample Source
Other, Spleen
 

Selection Method
Negative
 

Application
Cell Isolation
 

Brand
EasySep, RoboSep
 

Area of Interest
Immunology

Product Advantages

- Fast and easy-to-use

- Up to 98% purity

- No columns required

- Untouched, viable cells

What's Included

- EasySep™ Mouse Naïve CD8+ T Cell Isolation Kit (Catalog #19858)

    - EasySep™ Mouse Naïve CD8+ T Cell Isolation Cocktail, 0.5 mL

    - EasySep™ Streptavidin RapidSpheres™ 50001, 1 mL

    - EasySep™ Mouse FcR Blocker (Catalog #18720), 0.1 mL

- RoboSep™ Mouse Naïve CD8+T Cell Isolation Kit (Catalog #19858RF)

    - EasySep™ Mouse Naïve CD8+ T Cell Isolation Cocktail, 0.5 mL

    - EasySep™ Streptavidin RapidSpheres™ 50001, 1 mL

    - EasySep™ Mouse FcR Blocker (Catalog #18720), 0.1 mL

    - RoboSep™ Buffer (Catalog #20104)

    - RoboSep™ Filter Tips (Catalog #20125)

Catalog #: 19858 (EasySep), 19858RF (RoboSep)

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.