Overview

Easily and efficiently isolate highly purified mouse natural killer (NK) cells (CD3-CD49b+) from single-cell suspensions of mouse splenocytes and other tissue samples by immunomagnetic negative selection, with the EasySep™ Mouse NK Cell Isolation Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep™ negative selection procedure, unwanted cells are labeled with antibody complexes and magnetic particles. The following unwanted cells are targeted for removal: CD4, CD5, Ly6G, CD8a, F4/80, CD3e, CD19, CD24, TCRgd, and 7-4. The magnetically labeled cells are then separated from the untouched desired NK cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube. Following magnetic cell isolation in as little as 25 minutes, the desired NK cells are ready for downstream applications such as flow cytometry, culture, or cell-based assays.

Magnet Compatibility
- EasySep™ Magnet (Catalog #18000)
- “The Big Easy” EasySep™ Magnet (Catalog #18001)
- EasyEights™ EasySep™ Magnet (Catalog #18103)
- RoboSep™-S (Catalog #21000)
 

Subtype
Cell Isolation Kits
 

Cell Type
NK Cells
 

Species
Mouse
 

Sample Source
Spleen
 

Selection Method
Negative
 

Application
Cell Isolation
 

Brand
EasySep, RoboSep
 

Area of Interest
Immunology

Product Advantages

- Fast and easy-to-use

- No columns required

- Untouched, viable cells

What's Included

- EasySep™ Mouse NK Cell Isolation Kit (Catalog #19855)

    - EasySep™ Mouse NK Cell Isolation Cocktail, 0.5 mL

    - EasySep™ Streptavidin RapidSpheres™ 50001, 1 mL

- RoboSep™ Mouse NK Cell Isolation Kit (Catalog #19855RF)

    - EasySep™ Mouse NK Cell Isolation Cocktail, 0.5 mL

    - EasySep™ Streptavidin RapidSpheres™ 50001, 1 mL

    - RoboSep™ Buffer (Catalog #20104)

    - RoboSep™ Filter Tips (Catalog #20125)

Catalog #: 19855 (EasySep), 19855RF (RoboSep)

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.