Overview

Easily and efficiently isolate highly purified mouse pan-B cells (CD19+, CD19+CD138+, and CD138+), including conventional B-2 B cells, B-1 B cells and plasma cells from single-cell suspensions of splenocytes or other tissues samples by immunomagnetic negative selection, with the EasySep™ Mouse Pan-B Cell Isolation Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep™ negative selection procedure, unwanted cells are labeled with antibody complexes and magnetic particles. The following unwanted cells are targeted for removal: CD4, CD8, CD11c, CD49b, CD90.2, Ly-6C/G (Gr-1) and TER119. The magnetically labeled cells are then separated from the untouched desired pan-B cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube. Following magnetic cell isolation in as little as 10 minutes, the desired pan-B cells are ready for downstream applications such as flow cytometry, culture, or cell-based assays.

This kit is compatible for use with cells from disease models where the malignant cells (B-CLL) express CD43 or CD11b. For isolation of conventional B cells only, we recommend using the EasySep™ Mouse B Cell Isolation Kit (Catalog #19854).

Magnet Compatibility
- EasySep™ Magnet (Catalog #18000)
- “The Big Easy” EasySep™ Magnet (Catalog #18001)
- RoboSep™-S (Catalog #21000)
 

Subtype
Cell Isolation Kits
 

Cell Type
B Cells
 

Species
Mouse
 

Sample Source
Other, Spleen
 

Selection Method
Negative
 

Application
Cell Isolation
 

Brand
EasySep, RoboSep
 

Area of Interest
Immunology

Product Advantages

- Fast and easy-to-use

- Up to 98% purity

- No columns required

- Untouched, viable cells

What's Included

- EasySep™ Mouse Pan-B Cell Isolation Kit (Catalog #19844)

    - EasySep™ Mouse Pan-B Cell Isolation Cocktail, 0.5 mL

    - EasySep™ Streptavidin RapidSpheres™ 50001, 1 mL

- RoboSep™ Mouse Pan-B Cell Isolation Kit (Catalog #19844RF)

    - EasySep™ Mouse Pan-B Cell Isolation Cocktail, 0.5 mL

    - EasySep™ Streptavidin RapidSpheres™ 50001, 1 mL

    - RoboSep™ Buffer (Catalog #20104)

    - RoboSep™ Filter Tips (Catalog #20125)

Catalog #: 19844 (EasySep), 19844RF (RoboSep)

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.