Overview

The EasySep™ Mouse Plasmacytoid DC Isolation Kit is designed to isolate plasmacytoid dendritic cells (pDCs) from single-cell suspensions of splenocytes or other tissues by negative selection. Unwanted cells are targeted for removal with biotinylated antibodies that are directed against non-pDCs. Labeled cells are then recognized by Tetrameric Antibody Complexes that are directed against biotin and dextran. These cells are bound to magnetic particles and separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.

Magnet Compatibility
- EasySep™ Magnet (Catalog #18000)
- “The Big Easy” EasySep™ Magnet (Catalog #18001)
- RoboSep™-S (Catalog #21000)
 

Subtype
Cell Isolation Kits
 

Cell Type
Dendritic Cells
 

Species
Mouse
 

Sample Source
Spleen
 

Selection Method
Negative
 

Application
Cell Isolation
 

Brand
EasySep, RoboSep
 

Area of Interest
Immunology

Product Advantages

- Fast, easy-to-use and column-free

- Up to 94% purity

- Isolated cells are untouched

What's Included

- EasySep™ Mouse Plasmacytoid DC Isolation Kit (Catalog #19764)

    - EasySep™ Mouse Plasmacytoid DC Isolation Cocktail, 1 mL

    - EasySep™ Biotin Selection Cocktail, 2 x 1 mL

    - EasySep™ Magnetic Particles, 4 x 1 mL

- RoboSep™ Mouse Plasmacytoid DC Isolation Kit (Catalog #19764RF)

    - EasySep™ Mouse Plasmacytoid DC Isolation Cocktail, 1 mL

    - EasySep™ Biotin Selection Cocktail, 2 x 1 mL

    - EasySep™ Magnetic Particles, 4 x 1 mL

    - RoboSep™ Buffer (Catalog #20104) x 2

    - RoboSep™ Filter Tips (Catalog #20125) x 2

Catalog #: 19764 (EasySep), 19764RF (RoboSep)

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.