Overview

Easily isolate highly purified mouse CD45+ tumor-infiltrating leukocytes (TILs) by immunomagnetic positive selection using the EasySep™ Mouse TIL (CD45) Positive Selection Kit. This kit has been optimized for use on single-cell suspensions of solid mouse tumors, including tumors induced by implantation of 4T1, B16-F10, and CT26.WT cell lines into syngeneic mice. Due to the heterogeneity of mouse tumors, this kit may require optimization. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep™ positive selection procedure, desired cells are labeled with antibody complexes recognizing CD45 and magnetic particles. Labeled cells are separated using an EasySep™ magnet and by simply pouring or pipetting off the unwanted cells. The cells of interest remain in the tube. Following magnetic cell isolation, the desired CD45+ TILs are ready for downstream applications such as flow cytometry, culture, and cell-based experiments.

Magnet Compatibility
- EasySep™ Magnet (Catalog #18000)
- “The Big Easy” EasySep™ Magnet (Catalog #18001)
- EasyEights™ EasySep™ Magnet (Catalog #18103)
 

Subtype
Cell Isolation Kits
 

Cell Type
Leukocytes
 

Species
Mouse
 

Sample Source
Other, Tumor
 

Selection Method
Positive
 

Application
Cell Isolation
 

Brand
EasySep
 

Area of Interest
Cancer, Immunology

Product Advantages

- Fast, easy to use, and column-free

- Optimized for tumors and tissue samples with a low CD45 start frequency

- Flexible protocols for higher purity or recovery

What's Included

- EasySep™ Mouse TIL (CD45) Positive Selection Cocktail Component A, 0.25 mL

- EasySep™ Mouse TIL (CD45) Positive Selection Cocktail Component B, 0.25 mL

- EasySep™ Mouse TIL (CD45) Positive Selection Cocktail Component C, 1.5 mL

- EasySep™ Dextran RapidSpheres™ 50100, 2 x 1 mL

Catalog #: 100-0350

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.