Overview

The EasySep™ Release Human Biotin Positive Selection Kit is designed to isolate cells that are labeled with biotin-conjugated antibodies from fresh or previously frozen peripheral blood mononuclear cells or washed leukapheresis samples by immunomagnetic positive selection.

Desired cells are labeled with antibodies and magnetic particles, and separated without columns using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Then, bound magnetic particles are removed from the EasySep™-isolated, Biotin-antibody labeled cells, which are immediately available for downstream applications. Following cell isolation with this EasySep™ Release kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins or other chemically related ligands.

This product replaces the EasySep™ Human Biotin Positive Selection Kit (Catalog #18553), providing highly purified particle-free cells.

Magnet Compatibility
- EasySep™ Magnet (Catalog #18000)
- “The Big Easy” EasySep™ Magnet (Catalog #18001)
- EasyPlate™ Magnet (Catalog #18102)
- EasyEights™ Magnet (Catalog #18103)
 

Subtype
Cell Isolation Kits
 

Cell Type
B Cells, Dendritic Cells, Granulocytes and Subsets, Hematopoietic Stem and Progenitor Cells, Macrophages, Marrow Stromal Cells, Mesenchymal Stem and Progenitor Cells, Monocytes, Mononuclear Cells, Myeloid Cells, NK Cells, Other, Plasma, T Cells
 

Species
Human
 

Sample Source
Leukapheresis, Other, PBMC
 

Selection Method
Positive
 

Application
Cell Isolation
 

Brand
EasySep
 

Area of Interest
Immunology

Product Advantages

- Highly purified cells labeled with biotinylated antibodies isolated from human tissue in less than 40 minutes

- No-wash removal of EasySep™ Releasable RapidSpheres™

What's Included

- EasySep™ Release Human Biotin Positive Selection Kit (Catalog #17653)

    - EasySep™ Biotin Positive Cocktail, 0.5 mL

    - EasySep™ Releasable RapidSpheres™ 50201, 1 mL

    - EasySep™ Release Buffer (Concentrate), 3 x 1 mL

    - Anti-Human CD32 Blocker, 1 mL

Catalog #: 17653

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.