Overview

Easily isolate highly purified and magnetic particle-free human CD3+ cells from fresh or previously frozen peripheral blood mononuclear cells (PBMCs) or washed leukapheresis samples by immunomagnetic positive selection, with the EasySep™ Release Human CD3 Positive Selection Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep™ positive selection procedure, desired cells are first labeled with antibody complexes recognizing CD3 and magnetic particles called EasySep™ Releasable RapidSpheres™. Unlike traditional magnetic particles, which stay bound to the target cells, these RapidSpheres™ have a releasable feature. After separation using an EasySep™ magnet, bound magnetic particles are removed from the EasySep™-isolated CD3+ cells using a release agent, and unwanted non-CD3+ cells are targeted for depletion. The final isolated fraction contains highly purified CD3+ cells that are immediately ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction. Following cell isolation with this EasySep™ kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins, or other chemically related ligands. The CD3 antigen is expressed on all T cells and CD56+ NKT cells.

This kit is designed for cell therapy research applications, but may be qualified for use as an ancillary material following the framework outlined in USP<1043>. STEMCELL can work with you to qualify this reagent as an ancillary material under an approved investigational new drug (IND) or clinical trial application (CTA).

Magnet Compatibility
- EasySep™ Magnet (Catalog #18000)
- “The Big Easy” EasySep™ Magnet (Catalog #18001)
- EasyPlate™ Magnet (Catalog #18102)
- EasyEights™ Magnet (Catalog #18103)
- RoboSep™-S (Catalog #21000)
 

Subtype
Cell Isolation Kits
 

Cell Type
T Cells
 

Species
Human
 

Sample Source
Leukapheresis, PBMC
 

Selection Method
Positive
 

Brand
EasySep, RoboSep
 

Area of Interest
Immunology, Cell Therapy Development

Product Advantages

- Highly purified human CD3+ cells isolated in less than 30 minutes

- No-wash removal of EasySep™ Releasable RapidSpheres™

What's Included

- EasySep™ Release Human CD3 Positive Selection Kit (Catalog #17751)

    - EasySep™ Release Human CD3 Positive Selection Cocktail, 1 mL

    - EasySep™ Releasable RapidSpheres™ 50201, 1 mL

    - EasySep™ Release Buffer (Concentrate), 3 x 1 mL

- RoboSep™ Human CD3 Positive Selection Kit (Catalog #17751RF)

    - EasySep™ Release Human CD3 Positive Selection Cocktail, 1 mL

    - EasySep™ Releasable RapidSpheres™, 1 mL

    - EasySep™ Release Buffer (Concentrate), 3 x 1 mL

    - RoboSep™ Buffer (Catalog #20104)

    - RoboSep™ Filter Tips (Catalog #20125)

Catalog #: 17751 (EasySep), 17751RF (RoboSep)

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.