Overview

The EasySep™ Release Human CD4 Positive Selection Kit is designed to isolate CD4+ cells from fresh or previously frozen peripheral blood mononuclear cells or washed leukapheresis samples by immunomagnetic positive selection.

Desired cells are labeled with antibodies and magnetic particles, and separated without columns using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Then, bound magnetic particles are removed from the EasySep™-isolated CD4+ cells, which are immediately available for downstream applications. Following cell isolation with this EasySep™ Release kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins or other chemically related ligands. The CD4 antigen is strongly expressed on helper T cells and is expressed more weakly on peripheral blood monocytes and tissue macrophages.

Magnet Compatibility
- EasySep™ Magnet (Catalog #18000)
- “The Big Easy” EasySep™ Magnet (Catalog #18001)
- EasyPlate™ Magnet (Catalog #18102)
- EasyEights™ Magnet (Catalog #18103)
- RoboSep™-S (Catalog #21000)
 

Subtype
Cell Isolation Kits
 

Cell Type
T Cells, T Cells, CD4+
 

Species
Human
 

Sample Source
Leukapheresis, PBMC
 

Selection Method
Positive
 

Brand
EasySep, RoboSep
 

Area of Interest
Immunology, Cell Therapy Development

Product Advantages

- Highly purified human CD4+ cells isolated in less than 30 minutes

- No-wash removal of EasySep™ Releasable RapidSpheres™

What's Included

- EasySep™ Release Human CD4 Positive Selection Kit (Catalog #17752)

    - EasySep™ Release Human CD4 Positive Selection Cocktail, 1 mL

    - EasySep™ Releasable RapidSpheres™ 50201, 1 mL

    - EasySep™ Release Buffer (Concentrate), 3 x 1 mL

- RoboSep™ Human CD4 Positive Selection Kit (Catalog #17752RF)

    - EasySep™ Release Human CD4 Positive Selection Cocktail, 1 mL

    - EasySep™ Releasable RapidSpheres™, 1 mL

    - EasySep™ Release Buffer (Concentrate), 3 x 1 mL

    - RoboSep™ Buffer (Catalog #20104)

    - RoboSep™ Filter Tips (Catalog #20125)

    - EasySep™ EasyTube™-14 (Catalog #20128)

Catalog #: 17752 (EasySep), 17752RF (RoboSep)

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.