Overview

Easily isolate highly purified and magnetic particle-free human CD45+ leukocytes from single-cell suspensions of primary human tissues and tumors (Catalog #100-0105), or tissues and tumor xenografts from humanized mice (Catalog #100-0107) samples, by immunomagnetic positive selection, with the EasySep™ Release Human CD45 Positive Selection Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep™ positive selection procedure, desired cells are first labeled with antibody complexes recognizing CD45 and magnetic particles called EasySep™ Releasable RapidSpheres™. Unlike traditional magnetic particles, which stay bound to the target cells, these RapidSpheres™ have a releasable feature. After separation using an EasySep™ magnet, bound magnetic particles are removed from the EasySep™-isolated CD45+ cells using a release agent, and unwanted cells are targeted for depletion. The final isolated fraction contains highly purified CD45+ cells that are immediately ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction. Following cell isolation with this EasySep™ kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins, or other chemically related ligands.

Magnet Compatibility
- EasySep™ Magnet (Catalog #18000)
- “The Big Easy” EasySep™ Magnet (Catalog #18001)
- EasyEights™ EasySep™ Magnet (Catalog #18103)
- EasyPlate™ EasySep™ Magnet (Catalog #18102)
- RoboSep™-S (Catalog #21000)
 

Subtype
Cell Isolation Kits
 

Cell Type
Leukocytes
 

Species
Human, Mouse
 

Sample Source
Other, Tumor
 

Selection Method
Positive
 

Application
Cell Isolation
 

Brand
EasySep, RoboSep
 

Area of Interest
Cancer, Immunology

Product Advantages

- Highly purified CD45+ cells isolated in less than 45 minutes

- No-wash removal of EasySep™ Releasable RapidSpheres™

What's Included

- EasySep™ Release Human CD45 Positive Selection Kit (Catalog #100-0105)

    - EasySep™ Release Human CD45 Positive Selection Cocktail, 0.5 mL

    - EasySep™ Releasable RapidSpheres™, 1 mL

    - EasySep™ Release Buffer (Concentrate), 6 x 1 mL

- RoboSep™ Release Human CD45 Positive Selection Kit (Catalog #100-0108)

    - EasySep™ Release Human CD45 Positive Selection Cocktail, 0.5 mL

    - EasySep™ Releasable RapidSpheres™, 1 mL

    - EasySep™ Release Buffer (Concentrate), 6 x 1 mL

    - RoboSep™ Buffer (Catalog #20104)

    - RoboSep™ Filter Tips (Catalog #20125)

    - EasySep™ EasyTube™-14 (Catalog #20128)

- EasySep™ Release Human CD45 Positive Selection Kit for Humanized Mice (Catalog #100-0107)

    - EasySep™ Release Human CD45 Positive Selection Cocktail, 0.5 mL

    - EasySep™ Releasable RapidSpheres™, 1 mL

    - EasySep™ Release Buffer (Concentrate), 6 x 1 mL

    - EasySep™ Mouse FcR Blocker, 0.5 mL

- RoboSep™ Release Human CD45 Positive Selection Kit for Humanized Mice (Catalog #100-0109)

    - EasySep™ Release Human CD45 Positive Selection Cocktail, 0.5 mL

    - EasySep™ Releasable RapidSpheres™, 1 mL

    - EasySep™ Release Buffer (Concentrate), 6 x 1 mL

    - EasySep™ Mouse FcR Blocker, 0.5 mL

    - RoboSep™ Buffer (Catalog #20104)

    - RoboSep™ Filter Tips (Catalog #20125) x 2

    - EasySep™ EasyTube™-14 (Catalog #20128) x 2

Catalog #: 100-0105 (EasySep for Human Samples), 100-0108 (RoboSep for Human Samples), 100-0107 (EasySep for Humanized Mouse Samples), 100-0109 (RoboSep for Humanized Mouse Samples)

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.