Overview

Easily isolate highly purified and magnetic particle-free cells labeled with biotin antibodies from mouse splenocytes, using immunomagnetic positive selection, with the EasySep™ Release Mouse Biotin Positive Selection Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.


This EasySep™ positive selection procedure involves labeling cells with antibody complexes recognizing biotin and magnetic particles called EasySep™ Releasable RapidSpheres™. Unlike traditional magnetic particles that stay bound to the target cells, RapidSpheres™ have a releasable feature. Desired cells are first labeled with antibodies and these specialized magnetic particles, before being separated, without columns, using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Then, bound magnetic particles are removed from the EasySep™-isolated, biotin-antibody labeled cells using a release agent. Following magnetic cell isolation in less than 40 minutes with this EasySep™ Release kit, the desired cells are immediately available for downstream applications such as flow cytometry, culture, or DNA/RNA extraction. Antibody complexes remain bound to the surface of the desired cells and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins, or other chemically related ligands.

Magnet Compatibility
- EasySep™ Magnet (Catalog #18000)
- “The Big Easy” EasySep™ Magnet (Catalog #18001)
- EasyPlate™ Magnet (Catalog #18102)
- EasyEights™ Magnet (Catalog #18103)
 

Subtype
Cell Isolation Kits
 

Cell Type
B Cells, Dendritic Cells, Granulocytes and Subsets, Hematopoietic Stem and Progenitor Cells, Innate Lymphoid Cells, Lymphocytes, Macrophages, Marrow Stromal Cells, Mesenchymal Stem and Progenitor Cells, Monocytes, Mononuclear Cells, Myeloid Cells, NK Cells, Other, Plasma, T Cells
 

Species
Mouse
 

Sample Source
Bone Marrow, Other, Spleen
 

Selection Method
Positive
 

Application
Cell Isolation
 

Brand
EasySep
 

Area of Interest
Immunology

Product Advantages

- Highly purified cells labeled with biotinylated antibodies isolated from mouse tissue in less than 40 minutes

- No-wash removal of EasySep™ Releasable RapidSpheres™

What's Included

- EasySep™ Release Mouse Biotin Positive Selection Kit (Catalog #17655)

    - EasySep™ Biotin Positive Cocktail, 0.5 mL

    - EasySep™ Releasable RapidSpheres™ 50201, 1 mL

    - EasySep™ Release Buffer (Concentrate), 3 x 1 mL

    - EasySep™ Mouse FcR Blocker (Catalog #18731), 0.5 mL

Catalog #: 17655

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.