Overview

Isolate magnetic particle-free CD138+ plasma cells and plasmablasts from single-cell suspensions of mouse splenocytes or bone marrow in as little as 37 minutes. CD138+ cells are differentiated B cells that can be used in antibody discovery research due to their ability to generate affinity-matured antibodies. While hybridoma fusions with mouse bone marrow and splenocytes typically require screening thousands of colonies, enriching for CD138+ plasma cells prior to hybridoma fusion can substantially decrease the screening burden to generate hybridomas.

To achieve higher lab efficiency for your antibody discovery research, purify CD138+ plasma cells and plasmablasts without columns by using immunomagnetic positive selection. Simply label cells with antibodies and magnetic particles, place in an EasySep™ cell separation magnet, then remove the bound magnetic particles from the isolated CD138+ cell fraction. CD138+ cells isolated from immunized mice are up to 95% pure and can be used with hybridoma generation protocols such as electrofusion.

Magnet Compatibility
- EasySep™ Magnet (Catalog #18000)
- “The Big Easy” EasySep™ Magnet (Catalog #18001)
- EasyEights™ EasySep™ Magnet (Catalog #18103)
- RoboSep™-S (Catalog #21000)
 

Subtype
Cell Isolation Kits
 

Cell Type
B Cells
 

Species
Mouse
 

Sample Source
Bone Marrow, Lymph Node, Other, Spleen
 

Selection Method
Positive
 

Application
Cell Isolation
 

Brand
EasySep, RoboSep
 

Area of Interest
Hybridoma Generation, Immunology, Infectious Diseases

Product Advantages

- Up to 95% purity of plasma cells from immunized mouse samples

- No-wash removal of EasySep™ Releasable RapidSpheres™

- Compatible with hybridoma generation protocols, including electrofusion

What's Included

- EasySep™ Release Mouse CD138 Positive Selection Kit (Catalog #100-0601)

    - EasySep™ Release Mouse CD138 Positive Selection Cocktail, 1 x 1 mL

    - EasySep™ Mouse FcR Blocker, 2 x 0.5 mL

    - EasySep™ Isolation Cocktail Enhancer, 1 x 1 mL

    - EasySep™ Releasable RapidSpheres™ 50201, 1 x 1 mL

    - EasySep™ Release Buffer (Concentrate), 3 x 1 mL

- RoboSep™ Release Mouse CD138 Positive Selection Kit (Catalog #100-1440)

    - EasySep™ Release Mouse CD138 Positive Selection Cocktail, 1 x 1 mL

    - EasySep™ Mouse FcR Blocker, 2 x 0.5 mL

    - EasySep™ Isolation Cocktail Enhancer, 1 x 1 mL

    - EasySep™ Releasable RapidSpheres™ 50201, 1 x 1 mL

    - EasySep™ Release Buffer (Concentrate), 3 x 1 mL

    - RoboSep™ Buffer (Catalog #20104)

    - RoboSep™ Filter Tips (Catalog #20125) x 2

    - EasySep™ EasyTube-14 (Catalog #20128) x 2

Catalog #: 100-0601 (EasySep), 100-1440 (RoboSep)

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.