Overview

Easily isolate highly purified and magnetic particle-free cells labeled with Phycoerythrin (PE)-conjugated antibodies from mouse splenocytes, using immunomagnetic positive selection and the EasySep™ Release Mouse PE Positive Selection Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

This EasySep™ positive selection procedure involves labeling cells bound to PE-conjugated antibodies with antibody complexes and EasySep™ Releasable RapidSpheres™. Unlike traditional magnetic particles that stay bound to the target cells, RapidSpheres™ have a releasable feature. Desired cells are first labeled with antibodies and these specialized magnetic particles, before being separated, without columns, using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Then, bound magnetic particles are removed from the EasySep™-isolated, PE antibody-labeled cells using a release agent. Following magnetic cell isolation with this EasySep™ Release kit, the desired cells are immediately available for downstream applications such as flow cytometry, culture, or DNA/RNA extraction. Antibody complexes remain bound to the surface of the desired cells and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins or other chemically related ligands.

Magnet Compatibility
- EasySep™ Magnet (Catalog #18000)
- “The Big Easy” EasySep™ Magnet (Catalog #18001)
- EasyPlate™ Magnet (Catalog #18102)
- EasyEights™ Magnet (Catalog #18103)
 

Subtype
Cell Isolation Kits
 

Cell Type
B Cells, Dendritic Cells, Granulocytes and Subsets, Hematopoietic Stem and Progenitor Cells, Macrophages, Marrow Stromal Cells, Mesenchymal Stem and Progenitor Cells, Monocytes, Mononuclear Cells, Myeloid Cells, NK Cells, Other, Plasma, T Cells
 

Sample Source
Bone Marrow, Other, Spleen
 

Selection Method
Positive
 

Application
Cell Isolation
 

Brand
EasySep
 

Area of Interest
Immunology

Product Advantages

- Highly purified cells labeled with PE-conjugated antibodies isolated from mouse tissue in less than 40 minutes

- No-wash removal of EasySep™ Releasable RapidSpheres™

What's Included

- EasySep™ Release Mouse PE Positive Selection Kit (Catalog #17656)

    - EasySep™ Release PE Positive Selection Cocktail, 1 mL

    - EasySep™ Releasable RapidSpheres™ 50201, 1 mL

    - EasySep™ Release Buffer (Concentrate), 3 x 1 mL

    - EasySep™ Mouse FcR Blocker (Catalog #18731), 0.5 mL

Catalog #: 17656

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.