Overview

The RosetteSep™ HLA Lymphoid Cell Enrichment Cocktail is designed to enrich lymphoid (CD3⁺) cells from whole blood by negative selection for lineage-specific chimerism analysis. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing CD16, CD19, CD20, CD36, CD56 and glycophorin A on red blood cells (RBCs). When centrifuged over a buoyant density medium such as RosetteSep™ DM-L (Catalog #15705) the unwanted cells pellet along with the RBCs. The purified lymphoid cells are present as a highly enriched population at the interface between the plasma and the buoyant density medium. The desired cells are immediately ready for chimerism analysis or other downstream applications.

Subtype
Cell Isolation Kits
 

Cell Type
T Cells
 

Species
Human
 

Sample Source
Buffy Coat, Whole Blood
 

Selection Method
Negative
 

Application
Cell Isolation, In Vitro Diagnostic
 

Brand
RosetteSep
 

Area of Interest
Chimerism, HLA, Immunology

Product Advantages

- Fast and easy-to-use

- Requires no special equipment or training

- Isolated cells are untouched

What's Included

- RosetteSep™ HLA Lymphoid Cell Enrichment Kit (Catalog #15271HLA)

    - RosetteSep™ HLA Lymphoid Cell Enrichment Cocktail, 5 x 2 mL

    - RosetteSep™ DM-L, (Catalog #15705) x 2

Catalog #: 15271HLA

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.