Overview

The RosetteSep™ Human CD45 Depletion Cocktail is designed to enrich epithelial circulating tumor cells (CTCs) from whole blood by depleting CD45+ cells. Unwanted cells are targeted for depletion with Tetrameric Antibody Complexes recognizing CD45, CD66b and glycophorin A on red blood cells (RBCs). When centrifuged over a buoyant density medium such as Lymphoprep™ (Catalog #18060), the unwanted cells pellet along with the RBCs. The purified epithelial tumor cells are present as a highly enriched population at the interface between the plasma and the buoyant density medium.

Subtype
Cell Isolation Kits
 

Cell Type
Cancer Cells and Cell Lines
 

Species
Human
 

Sample Source
Buffy Coat, Whole Blood
 

Selection Method
Depletion
 

Application
Cell Isolation
 

Brand
RosetteSep
 

Area of Interest
Cancer, Immunology, Stem Cell Biology

Product Advantages

- Fast and easy-to-use

- Requires no special equipment or training

- Untouched, viable cells

- Can be combined with SepMate™ for consistent, high-throughput sample processing

What's Included

- RosetteSep™ Human CD45 Depletion Cocktail (Catalog #15122)

    - RosetteSep™ Human CD45 Depletion Cocktail, 2 mL

- RosetteSep™ Human CD45 Depletion Cocktail (Catalog #15162)

    - RosetteSep™ Human CD45 Depletion Cocktail, 5 x 2 mL

Catalog #: 15122 (40 mL), 15162 (200 mL)

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.