Overview

The RosetteSep™ Human Progenitor Cell Basic Pre-Enrichment Kit is designed to pre-enrich hematopoietic progenitor cells from cord blood by negative selection. Unwanted cells are targeted for removal with antibody complexes recognizing CD3, CD11b, CD19 and glycophorin A on red blood cells (RBCs). When centrifuged over a buoyant density medium such as Lymphoprep™ (a href="https://www.stemcell.com/products/lymphoprep.html">Catalog #18060), the unwanted cells pellet along with the RBCs. The hematopoietic progenitor cells are present as an enriched population at the interface between the plasma and the buoyant density medium.

Cells of interest can then be positively selected from the pre-enriched cell population using EasySep™ (e.g. EasySep™ Human CD34 Positive Selection).

Subtype
Cell Isolation Kits
 

Cell Type
Hematopoietic Stem and Progenitor Cells
 

Species
Human
 

Sample Source
Cord Blood
 

Selection Method
Negative
 

Application
Cell Isolation
 

Brand
RosetteSep
 

Area of Interest
Immunology, Stem Cell Biology
 

Product Advantages

- Fast and easy-to-use

- Requires no special equipment or training

- Untouched, viable cells

- Can be combined with SepMate™ for consistent, high-throughput sample processing

What's Included

- RosetteSep™ Human Progenitor Cell Basic Pre-Enrichment Kit (Catalog #15226)

    - RosetteSep™ Human Progenitor Cell Basic Pre-Enrichment Cocktail, 2 x 2.5 mL

- RosetteSep™ Human Progenitor Cell Basic Pre-Enrichment Cocktail (Catalog #15266)

    - RosetteSep™ Human Progenitor Cell Basic Pre-Enrichment Cocktail, 10 x 2.5 mL

Catalog #: 15226 (1000 mL), 15266 (5000 mL)

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.