Product Overview

The OsteoImage™ Mineralization Assay is a rapid, fluorescent in vitro assay for assessing bone cell mineralization. The OsteoImage^TM Assay can quantitate in vitro mineralization by osteogenic stem cells, primary osteoblasts, and osteoblast-like cell lines. It is based on specific binding of the fluorescent OsteoImage™ Staining Reagent to the hydroxyapatite portion of bone-like nodules deposited by cells. 

Unlike typical histochemical methods such as von Kossa and Alizarin red, neither of which is hydroxyapatite specific, the OsteoImage™ Assay eliminates multiple steps or tedious extraction steps.

Increase the speed, sensitivity and ease of measuring mineralization in your cell cultures with the OsteoImage™ Mineralization Assay.

Benefits

• Delivers qualitative visual fluorescent microscopy or quantitative fluorescent plate reader results

• Can be used with primary osteoblasts, osteoblast stem cells, and osteoblast cell lines

• Measures hydroxyapatite, similar to real bone

• Completed in less than 90 minutes, without tedious extractions

• Sensitive enough to detect time dependent increases in mineralization in differentiating cells

• Scalable for use in 6-well to 96-well plates

Content

• 5 x 96 well plates

• 1 x 500 μl OsteoImage™ Staining Reagent

• 1 x 50 mL Staining Reagent Dilution Buffer

• 1 x 50 mL OsteoImage™ Wash Buffer (10x)

Cryopreserved Rat Osteoblasts thawed, cultured and stained with OsteoImage™ Assay for mineralization at Day 24 (5 × 96-wells)

Catalog #: PA-1503

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Instructions for proper use of the OsteoImage™ Mineralization Assay