Product Overview

The ToxiLight™ 100% Lysis Reagent set is intended for use with the ToxiLight™ Non-destructive Cytotoxicity Assay (sold seperately) and is designed to give the user a 100% lysis or total adenylate kinase control. It contains the 100% Lysis Reagent, 10 ml and Tris-Acetate Buffer, 50 ml (required volume correction for sample wells), and is suitable for 200 tests in a 96 well format.

Simply add the 100% Lysis Reagent to the control wells at a ratio of 1:2 (lysis reagent : sample volume), volume correct the remaining sample wells with Tris-Acetate Buffer and then incubate at room temperature for 10 minutes.

The ToxiLight™ 100% Lysis Reagent should be included as a control at each time point within your cytotoxicity assay. After addition proceed with the standard ToxiLight™ Assay protocol.

Benefits

• Highly sensitive - The ToxiLight™ BioAssay Kit exploits the cyclic nature of the AK reaction whereby a small amount of the AK enzyme can generate high concentrations of ATP. This enables the detection of very subtle changes in cytotoxicity and reduces false negatives.

• Non-destructive - ToxiLight™ assay detects cellular AK present in cell culture supernatants, eliminating the need to lyse cells to perform the assay, and allowing multiple tests to be performed on the same sample.

• Simple - Assay requires the simple addition of a single reagent, which can be added directly to wells in which cells are growing, or to a small sample of aspirated culture supernatant.

• Fast - Readable signal is detectable 5 minutes after the addition of the ToxiLight™ BioAssay reagent.

• Flexible - Cell supernatants can be frozen with no loss of AK activity, allowing for long-term kinetic studies.

• Safe - No hazardous chemicals are required.

Content

ToxiLight™ 100% lysis reagent set (Sufficient for 200 tests)

Catalog #: LT07-517

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Instructions for proper use of the ToxiLight™ 100% lysis reagent set