Product Overview

Poietics™ Normal Human Preadipocytes (HprAD) are isolated from subcutaneous or visceral fat from normal (non-diabetic) donors. Preadipocytes from diabetic donors are available as a separate product. Pertinent additional donor information, including Body Mass Index (BMI) may be available by contacting Scientific Support.

Cryopreserved Poietics™ Normal Human Preadipocytes (HprAD) are guaranteed to contain ≥1 million or ≥4 million (subcutaneous only) cells per ampoule and test positive for adipogenic differentiation. All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.

Recommended Media and Reagents

• PGM™ -2 Preadipocyte Growth Medium-2 BulletKit™ Medium (provides both a proliferation medium and a differentiation medium guaranteed to induce differentiation into mature, functionally active adipocytes; Each bottle of the PGM-2™ BulletKit™ Medium contains enough differentiation medium to fully differentiate 10 million preadipocytes [10 complete 96-well plates] into mature, functionally active adipocytes).

The PGM™-2 Medium is offered as a BulletKit™ Medium (catalog no. PT-8002) which includes both the basal medium and the necessary supplements for both proliferation and differentiation of preadipocytes. The basal medium (PGM™-2 Basal Medium; catalog no. PT-8202) and the necessary supplements (PGM™-2 SingleQuot™ Kit; catalog no. PT-9502) can also be purchased individually.

Lonza guarantees the performance of Clonetics™/Poietics™ cells only if appropriate Clonetics™/Poietics™ media and reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees.

Benefits

• Test negative for mycoplasma, bacteria, yeast, and fungi

• HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots

• A Certificate ofAnalysis is provided for each cell lot purchased

Content

 1 x Cryopreserved ampule of Preadipocytes containing ≥1 million/ 4 million cells

Recommended Medium

Lonza PGM-2™ Preadipocyte Growth Medium-2 BulletKit™

Lonza PBM™ Preadipocyte Basal Medium (Insulin free)

Catalog #: PT-5020 -- ≥1 million cells

Catalog #: PT-5001 -- ≥4 million cells

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Instructions - Poietics™ Human Subcutaneous Preadipocytes

Reference guidefor human and animal cells from normal to diseased, fresh and cryopreserved