Features & Benefits

• 2x concentrated reagents minimize pipetting steps, simplify reaction assembly and improve accuracy

• Superior assay sensitivity and specificity with ultrapure AccuStart enzyme technology – maximum-yielding Taq DNA polymerase mutant controlled by stringent, multi-epitope antibody hot start

• Supports efficient vortex mixing with proprietary anti-foaming formulation

• FastMix formulation supports both fast and standard thermal cycling conditions

PerfeCTa SYBR Green FastMix is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

Description

PerfeCTa SYBR^® Green FastMix is a 2X concentrated, ready-to-use reaction cocktail that contains all components, except primers and DNA template. This rigorously optimized master mix contains of proprietary buffer technology, stabilizers and AccuFast Taq DNA polymerase to deliver maximum assay precision, sensitivity, and PCR efficiency for accelerated or conventional thermal cycling conditions for SYBR Green detection. Dye-based detection methods are critically dependent on highly specific amplification because dsDNA dyes will bind to any amplicon, including off-target primer elongation and primer dimerization. AccuFast hot start Taq DNA polymerase contains a proprietary mixture of ultra-pure monoclonal antibodies that stringently suppress primer elongation prior to the initial PCR denaturation step and allows for setup and multi-day storage at ambient room temperature prior to thermal cycling. AccuFast provides rapid release of fully active enzyme to support accelerated thermal cycling conditions.

Contents

Single-tube, 2X concentrated reagent containing:

• Reaction buffer with optimized concentrations of molecular-grade MgCl2, dATP, dCTP, dGTP, and dTTP.

• AccuStart II Taq DNA Polymerase

• SYBR Green I dye

• Proprietary enzyme stabilizers and performance-enhancing additives.

• Titrated reference dye (if applicable)

Storage & Handling

Store components in a constant temperature freezer at -25°C to -15°C protected from light upon receipt.
After thawing, mix thoroughly before using.
For lot specific expiry date, refer to package label, Certificate of Analysis or Product Specification Form.

Catalog #: 95074-250 -- 250 x 20 μL rxns (2 x 1.25 mL)

Catalog #: 95074-012 -- 1250 x 20 μL rxns (10 x 1.25 mL)

Catalog #: 95074-05K -- 5000 x 20 μL rxns (1 x 50 mL)

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Manual PerfeCTa SYBR Green FastMix, Low ROX